|
|||||
|
|
| 大连湾和辽东湾夏季海水细菌多样性16S rDNA分析 | |
| 引用本文： | 赫崇波,丛林林,高祥刚,仲禾,姜北,邓欢.大连湾和辽东湾夏季海水细菌多样性16S rDNA分析[J].水产科学,2008,27(9). |
| 作者姓名： | 赫崇波 丛林林 高祥刚 仲禾 姜北 邓欢 |
| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 2. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023;辽宁师范大学生命科学学院,辽宁,大连,116029 3. 辽宁出入境检验检疫局,辽宁,大连,116001 |
| 基金项目： | 国家海洋908项目辽宁专项，国家海洋局公益项目 |
| 摘 要： | 通过构建大连湾(DL17)和辽东湾(ZD-LDW017)2个站点夏季海水的细菌16s rDNA文库,利用16S rDNA序列比对法,对大连湾和辽东湾夏季海水细菌多样性进行了分析.试验结果表明,这2个海域的细菌主要为非培养细菌.每个海区分别随机选取了200个克隆进行酶切谱型分析和序列测定,共得到104个不同的DNA序列,其中大连湾51个序列,分别属于13种(属);辽东湾53个序列,分别属于13种(属).有6种菌为这2个海区所共有,这2个海区各自有不同的优势菌,且均未检测到致病菌,研究结果表明大连湾和辽东湾海水细菌具有丰富的多样性. |
| 关 键 词： | 多样性 非培养细菌 |
16S rDNA Analysis of Bacterial Diversity in Dalian Bay and Liaodong Bay during Summer |
|
| HE Chong-bo,CONG Lin-lin,GAO Xiang-gang,ZHONG He,JIANG Bei,DENG Huan.16S rDNA Analysis of Bacterial Diversity in Dalian Bay and Liaodong Bay during Summer[J].Fisheries Science,2008,27(9). | |
| Authors: | HE Chong-bo CONG Lin-lin GAO Xiang-gang ZHONG He JIANG Bei DENG Huan |
| Abstract: | The diversity of bacteria were analyzed in Dalian Bay and Liaodong Bay in Bohai sea during summer by constructing 16S rDNA libraries of bacterial samples collected from two survey stations(ZD-DL17) and(ZD-LDW017).The results showed that there were mainly uncultured bacteria in the two stations.There were 51 unique sequences belonging to 13 bacterial species(genera) in Dalian Bay and and in Liaodong Bay there were 53 unique sequences belonging to 13 bacteria species(genera).Six bacteria were shared by two stations and different predominant bacteria existed in the different stations,and no pathogenic bacterial species were found in both stations,indicating that the bacterial diversity existed in Dalian bay and Liaodong Bay. |
| Keywords: | 16S rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |