| 白菜GLDH 基因cDNA的克隆及序列分析 |
| |
| 引用本文： | 任锡亮,侯喜林,李英.白菜GLDH 基因cDNA的克隆及序列分析[J].园艺学报,2006,33(6):1345-1348. |
| |
| 作者姓名： | 任锡亮 侯喜林 李英 |
| |
| 作者单位： | ( 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095; 南京农业大学园艺学院, 江苏南京210095) |
| |
| 基金项目： | 国家自然科学基金资助项目(30500343) |
| |
| 摘 要： | 以白菜品种‘苏州青’ cDNA 为模板, 采用RT-PCR、巢式PCR、3’RACE和5’RACE技术, 获得了L - 半乳糖酸- 1, 4 - 内酯脱氢酶(EC 1.3.2.3, GLDH) 基因cDNA 2 034 bp全长序列。序列分析表明, GLDH基因cDNA序列编码601个氨基酸。其氨基酸序列与花椰菜GLDH基因具有98%的同源性, 与拟南芥GLDH基因具有90%的同源性。该基因在GenBank中登录号为AY899298。
|
| 关 键 词： | 白菜 L - 抗坏血酸 L - 半乳糖酸- 1 4 - 内酯脱氢酶 |
| 文章编号： | 0513-353X（2006）06-1345-04 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005-12-192006-04-13 |
Molecular Cloning and Sequence Analysis of GLDH Gene cDNA from Brassica campestris ssp. chinensis |
| |
| Ren Xiliang,Hou Xilin,Li Ying.Molecular Cloning and Sequence Analysis of GLDH Gene cDNA from Brassica campestris ssp. chinensis[J].Acta Horticulturae Sinica,2006,33(6):1345-1348. |
| |
| Authors: | Ren Xiliang Hou Xilin Li Ying |
| |
| Institution: | (National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, Jiangsu 210095, China; College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China) |
| |
| Abstract: | The L-galactono-1,4-lactone dehydrogenase(EC 1.3.2.3,GLDH)is a key enzyme that catalyzes the final step in the L-ascorbic acid synthetic pathway of plants.GLDH gene cDNA sequence was cloned with cDNA isolated from Brassica campestris ssp.chinensis'Suzhouqing'.A 2 034 bp full-length cDNA sequence of GLDH was obtained using RT-PCR,nested PCR,3'RACE and 5'RACE techniques.Further analysis on GLDH gene cDNA indicated that it encoded a peptide containing 601 amino acids.The amino acid sequence comparison with Brassica oleracea GLDH gene and Arabidopsis thaliana GLDH gene showed that identity was 98% and 90%,respectively.The sequence was accepted and released by GenBank(Accession number AY899298). |
| |
| Keywords: | Brassica campestris ssp chinensis Makino L-ascorbic acid L-galactono-1 4-lactone dehydrogenase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
