| 冬枣两个ACC氧化酶基因的cDNA克隆及其表达模式 |
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| 引用本文： | 魏绍冲,陈汝,赵相娟,张静.冬枣两个ACC氧化酶基因的cDNA克隆及其表达模式[J].园艺学报,2008,35(5):643-648. |
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| 作者姓名： | 魏绍冲 陈汝 赵相娟 张静 |
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| 作者单位： | (山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，山东泰安 271018；山东省果树研究所，山东泰安271000) |
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| 基金项目： | 山东农业大学校科研和教改项目 |
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| 摘 要： | 根据植物ACC氧化酶（ACO）家族的氨基酸和核苷酸保守区序列设计简并引物，采用3′RACE技术从半红期冬枣果实中分离了两个ACO同源基因片段，随后通过PCR技术进一步获得了两个包含完整开放阅读框（ORF）及3′端非编码区（UTR）序列的ACO基因的cDNA，即ZjACO1和ZjACO2。两个基因序列在GenBank中的登录号为EU216549和EU216550，其大小分别为1 115 bp和1 105 bp，编码蛋白大小分别为319个和321个氨基酸。ZjACO1和ZjACO2在核苷酸和氨基酸水平上的同源性分别为79.4%和84.0%。Northern杂交结果表明，这两个基因在冬枣叶片中不表达，而在不同发育阶段果实中的表达具有明显差异。
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| 关 键 词： | 冬枣 ACC氧化酶 果实 成熟 基因 克隆 表达 |
| 收稿时间： | 2007-10-29 |
Cloning and Expression Patterns of Two cDNAs Encoding ACC Oxidase in 'Dongzao'Jujube |
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| WEI Shao-chong,CHEN Ru,ZHAO Xiang-juan,ZHANG jing.Cloning and Expression Patterns of Two cDNAs Encoding ACC Oxidase in 'Dongzao'Jujube[J].Acta Horticulturae Sinica,2008,35(5):643-648. |
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| Authors: | WEI Shao-chong CHEN Ru ZHAO Xiang-juan ZHANG jing |
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| Institution: | （State Key Laboratory of Crop Biology，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai'an，Shandong 271018，China；Shandong Institute of Pomology, Tai'an，Shandong 271000，China） |
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| Abstract: | Two ACC oxidase（ACO）cDNA fragments were isolated from half-red‘Dongzao' jujube fruit by 3′ RACE technique using degenerate primers corresponding to the conserved nucleotide and amino acid region sequences of the ACO family, then the 5′ end encoding sequences of both cDNAs were obtained by RT-PCR experiments. The two cDNAs, named as ZjACO1 and ZjACO2, contain an ORF（open reading frame）with 3′UTR（untranslated region）respectively. Their accession numbers in GenBank are EU216549 and EU216550. The two cDNAs are 1 115 bp and 1 105 bp in length, while the predicted proteins of them are 319 and 321 aa long, respectively. The similarity between ZjACO1 and ZjACO2 is 79.4% at nucleotide level, and 84.0% at amino acid level. The result of Northern blot analysis indicated that expressions of two cDNAs in jujube leaves were both undetectable, while their expressions at the development and ripening stages of jujube fruit were obviously different. |
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| Keywords: | Ziziphus jujuba Ziziphus jujuba jujube ACC oxidase fruit ripening gene cloning expression |
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