| 桑下胚轴愈伤组织诱导及分化过程中内源激素的变化 |
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| 引用本文： | 张和禹,赵正龙.桑下胚轴愈伤组织诱导及分化过程中内源激素的变化[J].蚕业科学,2000,26(1):1-4. |
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| 作者姓名： | 张和禹 赵正龙 |
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| 作者单位： | 安徽农业大学蚕桑系,合肥,230036 |
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| 摘 要： | 以自然受精的桑种子的下胚轴为材料进行组织培养 ,MS +NAA( 1 0mg) +6 BA( 0 .5mg)为培养基诱导的愈伤组织生长正常 ,具有不定芽的分化能力。MS +2 ,4 D( 1 .0mg/L) +6 BA( 0 .5mg/L)诱导的愈伤组织没有分化能力 ,结构疏松并呈现褐变现象。愈伤组织分化培养与继代培养中 ,内源激素测定发现 :培养前期 ,前者的ABA、IAA和GA的含量均较后者保持一个较高的水平 ,而培养后期 ,后者的ABA有较大的上升 ,IAA和GA的含量迅速下降
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| 关 键 词： | 桑下胚轴 组织培养 愈伤组织 内源激素 |
Changes of Endogenous Hormones on Tissue Culture of Hypocotyl of Mulberry |
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| Zhang Heyu,Zhao Zhenglong.Changes of Endogenous Hormones on Tissue Culture of Hypocotyl of Mulberry[J].Acta Sericologica Sinica,2000,26(1):1-4. |
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| Authors: | Zhang Heyu Zhao Zhenglong |
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| Abstract: | Formation of adventitious
bud from callus derived from the hypocotyl of Mulberry seeds has been completed
successfully.Endogenous hormones content of calli was measured by ELISA.The calli induced
by MS＋NAA 1.0mg／L＋BA 0.5mg／L grew vigorously and had the ability of
differentiation.The content of IAA、ABA、and GA of which was higher than that of the
calli,which didn′t have the ability of differentiation,induced by MS＋2.4-D 1.0mg／L＋BA
0.5mg／L.The IAA、GA and ABA levels in calli cultured on medium for differentiation were
higher than that cultured on medium for subculture of callus during earlier culture
stage.While the level of ABA of the latter calli was significantly higher during later
stage,and the content of IAA and GA decreased rapidly. |
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| Keywords: | Hypocotyl of mulberry Tissue culture Callus Endogenous hormones |
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