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猪伪狂犬病PCR检测方法的建立与应用
引用本文:孙德刚,吴发兴,李晓成,黄保续,王洪斌,张燕霞.猪伪狂犬病PCR检测方法的建立与应用[J].中国动物检疫,2007,24(2):29-30.
作者姓名:孙德刚  吴发兴  李晓成  黄保续  王洪斌  张燕霞
作者单位:1. 中国动物卫生与流行病学中心,山东青岛,266032;东北农业大学,黑龙江哈尔滨,150030
2. 中国动物卫生与流行病学中心,山东青岛,266032
3. 东北农业大学,黑龙江哈尔滨,150030
摘    要:根据Genbank中PRV gD基因序列设计引物建立了检测猪场狂犬病的PCR方法并对某省25份猪伪狂犬病疑似病料进行检测,结果在2S份病料中,检出伪狂犬阳性病料7份,阳性率接近30%。通过检测结果及特异性和敏感性实验发现此检测方法敏感度很高。这种检测方法适合科学研究、临床诊断以及流行病学调查。

关 键 词:伪狂犬病病毒  检测
文章编号:1005-944X(2007)02-0029-02

Establishment and Application of PCR Method for Detection of Porcine Pseudorabies Virus
Sun DeGang,Wu FaXing,Li XiaoCheng,Huang BaoXu,Wang HongBin,Zhang YanXia.Establishment and Application of PCR Method for Detection of Porcine Pseudorabies Virus[J].China Journal Of Animal Quarantine,2007,24(2):29-30.
Authors:Sun DeGang  Wu FaXing  Li XiaoCheng  Huang BaoXu  Wang HongBin  Zhang YanXia
Institution:1.China Animal Health and Epidemiology Center, Qingdao,266032; 2.Northeast Agricultural University,Harbin
Abstract:According to PRV gD gene sequence in Genebank,a pair of primers were designed to develop a PCR and 25 suspicious samples from a province were detected using the developed method. Of 25,7 samples were positive. The positive rate of PRV approached 30%. It was concluded that the PCR method was of high sensitivity, and it could be applicable to research, clinical diagnosis and epidemiological investigation.
Keywords:PCR  GC buffer
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