| 红叶石楠离体快繁技术体系的建立与优化 |
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| 引用本文： | 侯春燕,任丽梅,张洁,王冬梅.红叶石楠离体快繁技术体系的建立与优化[J].河北农业大学学报,2007,30(5):44-47. |
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| 作者姓名： | 侯春燕 任丽梅 张洁 王冬梅 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 河北农业大学校科研和教改项目 |
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| 摘 要： | 以红叶石楠茎尖为外植体,探讨不同植物激素6-BA、IBA、NAA的浓度对试管苗增殖以及植株再生的影响,建立了一套红叶石楠离体培养技术体系。试验结果表明:红叶石楠初代培养采用MS+6-BA1.0 mg/L+NAA 0.1 mg/L培养基、茎尖继代培养采用MS+6-BA 2.0 mg/L和NAA 0.1 mg/L的培养基为宜,通过正交试验确定,植株增高选用MS+6-BA1.0+IBA 0.2 mg/L+NAA 0.2 mg/L的培养基效果较好,生根培养选用1/2MS+IBA1.0～1.5 mg/L培养基,生根率、移栽成活率可达100%。
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| 关 键 词： | 红叶石楠 组织培养 正交试验 快速繁殖 |
Establishment and optimization Photinia frasery rapid propagation system |
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| HOU Chun-yan,REN Li-mei,ZHANG Jie,WANG Dong-mei.Establishment and optimization Photinia frasery rapid propagation system[J].Journal of Agricultural University of Hebei,2007,30(5):44-47. |
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| Authors: | HOU Chun-yan REN Li-mei ZHANG Jie WANG Dong-mei |
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| Abstract: | Using the stem segment with single bud as the explant,this paper probes into the effects of different concentration 6-BA,NAA,IBA on proliferation and plantlet regeneration of Photinia frasery. The results showed that the appropriate medium for bud induction is MS+6-BA 1.0 mg/L+NAA0.1 mg/L,the medium of multiplication is MS+6-BA 2.0 mg/L+ NAA 0.1 mg/L.Using orthogonal tests to determine the medium for plant grow is MS+6-BA 1.0 mg/L+IBA 0.2 mg/L+NAA 0.2 mg/L,and the medium of rooting is 1/2 MS +IBA 1.0～1.5 mg/L,with up to 100% survival rate. |
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| Keywords: | Photinia frasery tissue culture orthogonal tests plantlet rapid propagation |
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