| 菊花18个品种的RAPD分析 |
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| 引用本文： | 秦贺兰,游捷,高俊平.菊花18个品种的RAPD分析[J].园艺学报,2002,29(5):488-490. |
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| 作者姓名： | 秦贺兰 游捷 高俊平 |
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| 作者单位： | (中国农业大学观赏园艺与园林系, 北京100094) |
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| 基金项目： | 农业部DUS测试项目资助 ( 95农 -11-0 1-0 7) |
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| 摘 要： | 采用RAPD 技术分析了18 个菊花品种DNA 的多态性。从80 个10 碱基随机引物中筛选出多态性频率高的3 个引物。扩增的多态性片段在600 bp～1300 bp 之间。检测出两个品种特有的分子标记, ‘大红托桂’有OPD15 (1200 bp) ,‘玉翎管’缺失OPA17 (1100 bp) 。瓣型一致的品种间基因型相似系数较高。
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| 关 键 词： | 菊花 RAPD 品种鉴定 相似系数 |
| 文章编号： | 0513-353X(2002)05-0488-03 |
| 修稿时间： | 2001年11月1日 |
RAPD Analysis of 18 Chrysanthemum Cuitivars |
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| Qin Helan,You Jie,and Gao Junping.RAPD Analysis of 18 Chrysanthemum Cuitivars[J].Acta Horticulturae Sinica,2002,29(5):488-490. |
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| Authors: | Qin Helan You Jie and Gao Junping |
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| Institution: | (Department of Ornamental Horticulture and Landscape Architecture , China Agricultural University , Beijing100094 , China) |
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| Abstract: | cultivars of Chrysanthemum were analyzed using random amplified polymorphic DNAs(RAPDs).Three primers out of 80 random primers showed high polymorphism,the polymorphic amplification fragment length ranged from 600 bp to 1300 bp. The 1200 bp fragment generated by primer OPD15 was present only in `Dahongtuogui', while the 1100 bp fragment generated by primer OPA17 was only absent in `yulingguan' in 18 cultivars.The cultivars with similar petal type have high similarity coeficience(SC)among 18 cultivars. |
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| Keywords: | Chrysanthemum RAPD Cultivars identification Similarity coefficence |
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