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| 人工感染的刺激隐核虫各期虫体的超微结构 | |
| 引用本文： | 黄玮.人工感染的刺激隐核虫各期虫体的超微结构[J].水产学报,2005,29(5):635-642. |
| 作者姓名： | 黄玮 |
| 作者单位： | 1. 中山大学生命科学学院,广东,广州,510275;广东省水产学校,广东,广州,510320 2. 中山大学生命科学学院,广东,广州,510275;西南农业大学动物科学学院,重庆,402460 3. 中山大学生命科学学院,广东,广州,510275 |
| 基金项目： | 国家自然科学基金资助（30440052）;广东省自然科学基金团队项目（20023002）;广东省自然科学基金面上项目（031640） |
| 摘 要： | 从天然感染的卵圆鲳鲹（Trachiruotus blochii）分离到1株刺激隐核虫（Cryptocaryon irritans），再经人工感染的方法收集各期虫体，制成电镜样品，对虫体进行超微结构研究。刺激隐核虫质膜的基本结构与淡水的多子小瓜虫的质膜相似，分为3层；外限制膜、外胞膜和内胞膜。刺激隐核虫的质膜小泡内充满大量电子致密物质（EDM）。幼虫、幼体和滋养体均具有复杂的口器，由胞咽、口肋、双动基体口纤毛和线带等组成，但不具备膜口类纤毛虫所具有的独特的细胞器：Lieberkuhn氏器。单动基体的体纤毛存在于幼虫、幼体和滋养体，但在包囊期，体纤毛和口器消失。各期虫体的胞质中具有线粒体、高尔基体和脂肪体等细胞器，有的阶段具有粘液囊、伸缩泡、食物泡、共生细菌等。粘液囊在大小、形态和分布上与多子小瓜虫的不同。文中分析了刺激隐核虫在形态上与多子小瓜虫的诸多差异，认为刺激隐核虫的分类更适合归于原口类（Promotome），而不是膜口类（Ophryoglenina）。 |
| 关 键 词： | 刺激隐核虫 超微结构 人工感染 |
| 文章编号： | 1000-0615（2005）05-0635-08 |
| 收稿时间： | 2004-12-20 |
| 修稿时间： | 2004年12月20 |
Ultrastructural study on Cryptocaryon irritans throughout the life cycle of artificially infecting Trachinotus blochi |
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| HUANG Wei.Ultrastructural study on Cryptocaryon irritans throughout the life cycle of artificially infecting Trachinotus blochi[J].Journal of Fisheries of China,2005,29(5):635-642. | |
| Authors: | HUANG Wei |
| Institution: | 1. School of life Science, Sun Yat-sen University, Guangzhou 510275, China; 2. Aquacultura! School of Guangdortg Province, Guangzhou 510320, China; 3. School of Animal Science, Southwest Agricultural University, Chongqing 402460, China |
| Abstract: | The morphology of an isolate of parasitic ciliate Cryptocaryon irritans obtained from cage-cultured snubnose pompano(Trachinotus blochii)from Huidong,Guangdong Province in China,was examined by observation of scanning electron microscopy(SEM) and transmission electron microscopy(TEM).The strains were maintained in experimentally infecting snubnose pompano from which the sample used for ultrastructural observation was collected.The general structural organization of the pellicles resembles that of the freshwater holotrich Ichthyophthirius multifiliis,consisting of three membranes:outer limiting membrane,the outer alveolar membrane and the inner alveolar membrane.The pellicular alveoli are characterized by the accumulation of electron dense material in the trophont,tomite and theront,this material being lost in the newly-encysted tomont.The theront, tomite and trophont possess a complex oral apparatus,being composed of cytopharynx,oral rib,dikinetids and nematodesmata,without an organelle of Lieberkuhn which is considered as a synapomorphy of the suborder Ophryoglenina.The monokinetid somatic cilia appear in rows longitudinally on the theronts,tomites and trophonts,but being lost at early stage of tomonts.There are mitochondria,Golgi apparatus and lipid droplets in the cytoplasma of all stages of parasite,and mucocysts,contractile vacuoles and endosymbiotic bacteria in theronts and trophonts.The size,shape and distribution of mucocysts in C.irritans are different from those of I.multifiliis.The present study suggests that the taxonomic affinities of C.irritans should lie with prostome ciliates by analysis of its different morphological characteristics from I.multifiliis. |
| Keywords: | Cryptocaryon irritans ultrastructure artificial infection |
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