| 次黄嘌呤对猪卵母细胞体外自发成熟抑制作用的研究 |
| |
| 引用本文： | 苏友强,夏国良,陈勇,赫晨,傅国栋.次黄嘌呤对猪卵母细胞体外自发成熟抑制作用的研究[J].畜牧兽医学报,2000,31(1):22-27. |
| |
| 作者姓名： | 苏友强 夏国良 陈勇 赫晨 傅国栋 |
| |
| 作者单位： | 1. 中国农业大学生物学院,北京,100094
2. 农业部饲料工业中心,北京,100094 |
| |
| 基金项目： | 国家自然科学基金!(396 70 531) |
| |
| 摘 要： | 利用猪卵母细胞体外无血清培养技术，研究了次黄嘌呤（ＨＸ）对猪卵母细胞体外自发成熟的抑制作用。猪卵丘－卵组细胞复合体（ＣＯＣ）和裸卵母细胞（ＤＯ）取自初情期猪卵巢，培养在Ｍ－１９９培养液中，并施以各种自理培养不同时间后，观察卵母细胞核成熟（ＧＶＢＤ）情况。实验结果表明：⑴ＨＸ（１－４mmol/Ｌ）对猪ＣＯＣ的自发成熟具有抑制 作用，且具有剂量领事关系。４mmol/Ｌ的ＨＸ对ＣＯＣ和ＤＯ自发成熟的抑制作用，且具
|
| 关 键 词： | 次黄嘌呤 抑制作用 猪 卵母细胞 自发成熟 |
STUDIES ON THE INHIBITORY EFFECT OF HYPOXANTHINE ON THE SPONTANEOUS MEIOTIC MATURATION OF PORCINE OOCYTES IN VITRO |
| |
| Su Youqiang,Xia Guoliang,Chen Yong,He Chen,Fu Guodong.STUDIES ON THE INHIBITORY EFFECT OF HYPOXANTHINE ON THE SPONTANEOUS MEIOTIC MATURATION OF PORCINE OOCYTES IN VITRO[J].Acta Veterinaria et Zootechnica Sinica,2000,31(1):22-27. |
| |
| Authors: | Su Youqiang Xia Guoliang Chen Yong He Chen Fu Guodong |
| |
| Abstract: | Using the serum-free culture technique for pig oocyte,the inhibitory effect of hypoxanthine (HX) on the spontaneous meiotic maturation of porcine oocytes was investigated.Pig cumulus oocyte complexes and denuded oocytes (COC and DO) were obtained from the ovaries of prepubertal gilts and cultured in medium 199.Different treatments were carried out on the oocytes during the culture.The incidence of germinal vesicle breakdown (GVBD) was examined at the end of the culture.The results showed:(1)HX,at the concentration of 1-4mmol/L,could significantly inhibit the spontaneous maturation of pig COC in a dose-dependent manner.The inhibitory effect of 4mmol/L HX on COC was not different from that on DO.(2)The inhibitory effect of HX (4mmol/L) on COC decreased in a time-dependent manner within the 72-hour culture period.(3)COC still could mature spontaneously in the following subsequent culture period when transferred from HX-supplemented medium to HX-free medium.These results indicated that HX could partially inhibit the spontaneous meiotic maturation of pig oocytes in vitro in a dose-dependent manner;This inhibitory effect is reversible. |
| |
| Keywords: | Hypoxanthine Inhibitory effect Pig oocytes Spontaneous maturation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
