| 地高辛标记大肠杆菌1型菌毛结构基因核酸探针对鸡大肠杆菌分离株的检测 |
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| 引用本文： | 戴鼎震,陆福军,龚大春,杨待建.地高辛标记大肠杆菌1型菌毛结构基因核酸探针对鸡大肠杆菌分离株的检测[J].畜牧与兽医,2000,32(4). |
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| 作者姓名： | 戴鼎震 陆福军 龚大春 杨待建 |
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| 作者单位： | 1. 江苏省农业科学院,江苏,南京,210014
2. 湖北农学院,湖北,荆州,434103 |
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| 摘 要： | 将PCR扩增的鸡大肠杆菌 1型菌毛蛋白结构基因 (pilA)用地高辛标记成核酸探针与分属 2 8个血清型的 50个鸡大肠杆菌分离株进行斑点杂交 ,阳性率达 84% ,用甘露糖敏感血凝试验 (MSHA)检测阳性率为 72 % ,表明核酸杂交比MSHA法更敏感。
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| 关 键 词： | 鸡大肠杆菌 1型菌毛结构基因 核酸探针 杂交 |
Detection of nucleotide probe of type 1 pili pilA gene of avian Escherichia coli labeled with digitoxin hybridizated to avian Escherichia coli isolated strain |
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| Dai Ding-zhen,Lu Fu-jun,Gong Da-chun,Yang Dai-jian.Detection of nucleotide probe of type 1 pili pilA gene of avian Escherichia coli labeled with digitoxin hybridizated to avian Escherichia coli isolated strain[J].Animal Husbandry & Veterinary Medicine,2000,32(4). |
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| Authors: | Dai Ding-zhen Lu Fu-jun Gong Da-chun Yang Dai-jian |
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| Abstract: | Type 1 pili pilA gene of avian Escherichia coli amplificated by PCR was labeled with digitoxin and used as nucleotide probe.50 avian Escherichia coli isolated strains samples including 28 serotype were choiced for hybridization with the nucleotide probe.The positive rate was 84% but the positive rate was 72% when detected with MSHA test.The results showed that nucleotide hybridization is more sensitive than MSHA test. |
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| Keywords: | avian Escherichia coli type 1 pili pilA gene nucleotide probe hybridization |
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