| 双酶法分解怀山药的工艺研究 |
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| 引用本文： | 刘瑞山,梁治军,常路路,王新房,齐强强.双酶法分解怀山药的工艺研究[J].安徽农业科学,2013(14):6462-6465. |
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| 作者姓名： | 刘瑞山 梁治军 常路路 王新房 齐强强 |
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| 作者单位： | 河南省淼雨饮品股份有限公司,河南焦作,454000 |
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| 摘 要： | 目的]研究高温α-淀粉酶和糖化酶对怀山药的分解效果。方法]利用高温α-淀粉酶和糖化酶对怀山药分步进行水解糖化以减小分散颗粒直径和沉淀率,在控制一定温度和时间的条件下,采用正交设计和因素分析来确定最适酶解条件,从而提高山药汁的可溶性固形物百分含量,降低其沉淀率和色泽。结果]试验表明,双酶法分解怀山药的最适酶解条件为:高温α-淀粉酶添加量0.4%(20 000U),山药浓度10%,酶解温度85℃,时间2 h条件下酶解效果最佳;糖化酶添加量0.4%(10 000 U),糖化温度55℃,时间3 h,pH 5.0。结论]研究可为怀山药的深度开发提供一定的理论依据。
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| 关 键 词： | 怀山药 高温α-淀粉酶 糖化酶 可溶性固形物含量 沉淀率 |
The Technology of Composite Amylases Hydrolyzing Huaiqing Yam |
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| Institution: | LIU Rui-shan et al (Henan Miaoyu Beverage Company Limited by Shares, Jiaozuo, Henan 454000) |
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| Abstract: | Objective] To study hydrolyzing effect of high temperature α-amylase and glucoamylase on Huaiqing yam. Method] High temper- ature a-amylase arid glucoamylase were used to conduct hydrolysis and saccharification, in order to reduce the dispersed particle diameter and sedimentation rate. Under the condition of controlling temperature and time, orthogonal design and factor analysis were adopted to determine the optimal hydrolysis conditions, so as to improve soluble solids content and reduce sedimentation rate and color. Result] The optimal hydrolysis conditions are： high temperature α-arnylase 0.4% （20 000 U）, yam 10%, temperature 85℃, time 2 h; Glucoamylase 0.4% （10 000 U）, tem- perature 55 ℃, time 3 h, pH 5.0. Conclusion ] The study can provide theoretical basis for deeply development of Huaiqing yam. |
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| Keywords: | Huaiqing yam High temperature α-amylase Glucoamylase Soluble solids content Sedimentation rate |
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