| 柽柳THWRKY12基因的克隆及表达分析 |
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| 引用本文： | 王敬雪,唐月坤,刘琪,梁春强,王玉成.柽柳THWRKY12基因的克隆及表达分析[J].安徽农业科学,2013(14):6147-6148,6187. |
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| 作者姓名： | 王敬雪 唐月坤 刘琪 梁春强 王玉成 |
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| 作者单位： | 东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨,150040;东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨,150040;东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨,150040;东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨,150040;东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨,150040 |
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| 基金项目： | 东北林业大学大学生创新实验项目 |
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| 摘 要： | 目的]克隆柽柳THWRKY12基因,并对其进行表达分析。方法]分别用400 mmol/L NaCl溶液、20%PEG及100μmol/L ABA对柽柳幼苗进行胁迫处理后,用RT-PCR技术研究THWRKY12基因在各组织中的表达情况。结果]在NaCl和PEG处理下,THWRKY12基因在柽柳幼苗各组织中表达总体呈上调趋势,表明该基因与柽柳的抗盐碱及抗旱过程有关;且ABA处理后THWRKY12基因的表达模式与前两者大致相同,表明该基因可能通过ABA调控的信号通路参与柽柳的抗盐碱及抗旱调控。结论]该研究为研究WRKY基因在柽柳抗逆性中的作用奠定了基础。
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| 关 键 词： | 柽柳 WRKY转录因子 RT-PCR 盐碱胁迫 干旱胁迫 |
Cloning and Expression Analysis of THWRKY12 Gene in Tamarix hispida |
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| Institution: | WANG Jing-xue et al ( State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040) |
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| Abstract: | Objective ] The paper described the cloning and analyses of the expression of THWRKY12 gene from Tamarix hispida. Method ] The T. hispida seedlings were treated with 400 mmol/L NaC1 solution ,20% PEG or 100μmol/L ABA respectively. Then the expression of THWRKY12 gene in different tissues was studied by using real-time RT-PCR technology. Result] The expression of THWRKY12 gene in different tissues of T. hispida seedlings showed an overall upregulation trend under both the treatment of NaC1 and PEG. It showed that the gene is involved in the process of saline - alkali resistance and drought resistance of T. hispida, treatments,indicating that the gene might involve in the regulation of sa- line stress tolerance and drought resistance of T. hispida through the ABA-dependant signaling pathway. Conclusion] The study laid a foundation for the study of the function of WRKY gene in the stress resistance of T. hispida. |
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