| 昆虫病原线虫rDNA多态性分析 |
| |
| 引用本文： | 丘雪红,韩日畴,许再福.昆虫病原线虫rDNA多态性分析[J].昆虫天敌,2003,25(3):130-144. |
| |
| 作者姓名： | 丘雪红 韩日畴 许再福 |
| |
| 作者单位： | [1]广东省昆虫研究所，广州510260 [2]华南农业大学资源环境学院，广州510642 |
| |
| 基金项目： | 欧盟第五框架国际合作项目 (DIABOLO)，国家科技部农业成果转化基金，广州市科技局星火计划项目的联合资助 |
| |
| 摘 要： | 本文对国内外昆虫病原线虫斯氏属和异小杆属的47个品系进行rDNA—ITS PCR—RFLP分析，研究其DNA多态性，并构建了分子系统发育树状图。各品系的ITS区无明显的长度差异，PCR—RFLP分析将47个品系分为斯氏属和异小杆属两大类，两属线虫又分别分为11组和4组。所得结果丰富了ITS PCR—RFLP图谱库，为弄清我国的昆虫病原线虫与国外种类的分子系统发育关系及未定名线虫的鉴定提供重要依据，同时为筛选适合的线虫种类防治害虫奠定基础。
|
| 关 键 词： | 昆虫病原线虫 rDNA多态性 分子系统发育树状图 核糖体DNA |
| 文章编号： | 1001-6155(2003)02-130-15 |
rDNA ITS POLYMORPHISM ANALYSIS OF ENTOMOPATHOGENIC NEMATODES |
| |
| QIU Xuehong,HAN Richou XU Zaifu.rDNA ITS POLYMORPHISM ANALYSIS OF ENTOMOPATHOGENIC NEMATODES[J].Natural Enemies of Insects,2003,25(3):130-144. |
| |
| Authors: | QIU Xuehong HAN Richou XU Zaifu |
| |
| Institution: | QIU Xuehong+1 HAN Richou+1 XU Zaifu+2 |
| |
| Abstract: | Entomopathogenic nematodes, Steinernema and Heterorhabditis are currently being mass-produced commercially and used to control many economically important pests as an effective biocontrol agent. The biological control potential of these nematodes requires rapid and reliable diagnostic methods for species identification. In this study, rDNA ITS polymorphism of 47 local and introduced entomopathogenic nematode isolates was analyzed by the rDNA ITS PCR-RFLP method, and a phylogenetic tree was constructed from the data obtained. No length variation was observed for ITS regions among the 47 isolates. However, variations in the RFLP patterns were recorded after digestion of the amplified ITS PCR products of various nematode isolates with nine restriction enzymes. Digestion patterns provided strong evidences for the separation of Steinernema and Heterorhabditis spp. From the patterns, the tested Steinernema and Heterorhabditis isolates contained eleven and four groups respectively. The results obtained enlarge the RFLP patterns, which is very useful by other researchers for the identification of entomopathogenic nematodes. |
| |
| Keywords: | Steinernema Heterorhabditis rDNA ITS PCR-RFLP DNA polymorphism |
| 本文献已被 CNKI 维普 等数据库收录！ |
