| 基于浸泡法的牙鲆耳石锶标记技术研究 |
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| 引用本文： | 司飞,任建功,王青林,孙朝徽,刘洪波,姜涛,杨健,王俊.基于浸泡法的牙鲆耳石锶标记技术研究[J].中国水产科学,2019,26(3):534-545. |
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| 作者姓名： | 司飞 任建功 王青林 孙朝徽 刘洪波 姜涛 杨健 王俊 |
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| 作者单位： | 1. 中国水产科学研究院北戴河中心实验站, 河北 秦皇岛 066100;2. 河北科技师范学院, 河北 秦皇岛 066600;3. 中国水产科学研究院淡水渔业研究中心, 江苏 无锡 214081;4. 中国水产科学研究院黄海水产研究所, 山东 青岛 266071;5. 青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东 青岛 266200 |
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| 基金项目： | 现代农业产业技术体系国家海水鱼产业技术体系（CARS-47-Z03）;公益性行业（农业）科研专项经费项目（201303050）. |
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| 摘 要： | 为了探究耳石微化学标记技术在牙鲆增殖放流中的应用,以全长(1.56±0.16)cm的牙鲆幼鱼为研究对象,在其养殖水中分别添加0.5 mg/L、2 mg/L、8 mg/L、32 mg/L SrCl_2·6H_2O,以无添加SrCl_2·6H_2O组为对照组,人为改变牙鲆幼鱼生存环境中Sr~(2+)浓度72 h,研究不同浓度SrCl_2·6H_2O对牙鲆耳石锶标记效果。标记期间每24 h换水1次,换水量为50%,换水后补加SrCl_2·6H_2O至原始浓度,记录各浓度组标记期间及标记后的鱼苗死亡数量。利用X射线电子探针微区分析仪(EPMA)对耳石样本进行分析,定量线分析结果表明, 0.5 mg/L、2 mg/L SrCl_2·6H_2O浸染不能形成牙鲆耳石锶标记。8 mg/L浓度组出现Sr峰区, Sr/Ca (Sr:Ca×1000)均值为4.50～6.60, Sr峰值为5.49～8.49。32 mg/L浓度组Sr峰区均值为4.83～7.55, Sr峰值为5.62～11.04。这说明8 mg/L、32 mg/L SrCl_2·6H_2O可对牙鲆耳石进行锶标记。但8 mg/L浓度组出现未标记样本,且Sr峰区均值较32 mg/L浓度组低。32 mg/L浓度组Sr/Ca比值显著增高,即锶标记的Sr/Ca比值为6.61±0.86,与标记前和对照组Sr/Ca比值差异均显著(P0.05),锶标记率100%。面分布分析(mapping analysis)结果表明,从8 mg/L SrCl_2·6H_2O浓度组开始,耳石出现红色"高锶标记环",与耳石本底颜色对比明显,且随浓度升高,红色"高锶标记环"由浅变深,锶标记效果明显增强。单因素方差分析和Duncan多重比较结果显示,各浓度组全长、体重与对照组差异不显著(P0.05),不同浓度的SrCl_2·6H_2O对牙鲆幼鱼生长无影响。经χ2检验,各浓度组在浸泡标记期间死亡率及标记后养殖90 d的累积死亡率无显著性差异(P0.05),不同浓度的SrCl_2·6H_2O对牙鲆幼鱼死亡率无显著影响。因此, 32 mg/L SrCl_2·6H_2O为最佳标记浓度。
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| 关 键 词： | 锶标记 耳石 牙鲆 SrCl2· 6H2O |
| 修稿时间： | 2019/5/20 0:00:00 |
Strontium marking on otoliths of Paralichthys olivaceus based on immersion experiments |
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| SI Fei,REN Jiangong,WANG Qinglin,SUN Zhaohui,LIU Hongbo,JIANG Tao,YANG Jian,WANG Jun.Strontium marking on otoliths of Paralichthys olivaceus based on immersion experiments[J].Journal of Fishery Sciences of China,2019,26(3):534-545. |
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| Authors: | SI Fei REN Jiangong WANG Qinglin SUN Zhaohui LIU Hongbo JIANG Tao YANG Jian WANG Jun |
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| Institution: | 1. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;2. Hebei Normal University of Science and Technology, Qinhuangdao 066600, China;3. Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;4. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;5. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China |
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| Abstract: | To explore the method of microchemistry marking on the otolith of Japanese flounder, Paralichthys olivaceus, during the process of proliferation and release, the juveniles with a total length of (1.56±0.16) cm were immersed at four different concentrations (0.5, 2, 8, and 32 mg/L) of SrCl2·6H2O for 72 h to detect the Sr sedimentation effect. During the immersion period, half of the water was exchanged by fresh seawater daily, and SrCl2·6H2O were added to maintain constant concentration for each group. The exogenous Sr sedimentation in otoliths was detected by X-ray electron probe microanalyzer (EPMA). Results of line transect analysis showed that the strontium marking on otoliths could not be formed after the immersion of two concentrations (0.5 mg/L and 2 mg/L). The Sr high area appeared in the 8 mg/L concentration group with a 5.49-8.49 peak value, and the mean value of the Sr/Ca (Sr:Ca×1000) ratio was 4.50-6.60. Furthermore, in the 32 mg/L group, the mean value of the Sr/Ca ratio was 6.61±0.86, and the peak value of Sr was 5.62-11.04. This means that otoliths of Paralichthys olivaceus can be marked with strontium by the method of SrCl2·6H2O immersion. However, in the 8 mg/L concentration group there were also unlabeled samples. and the mean value of Sr/Ca was lower than that of the 32 mg/L group. In the 32 mg/L group, the strontium marking rate was 100%, and the mean value of the Sr/Ca ratio was 6.61±0.86, which was significantly different (P < 0.05) compared with that of the control group. The EPMA mapping analysis showed that the red "high strontium marking ring" in the otolith appeared in the 8 mg/L group and 32 mg/L group, and the color of the "high strontium marking ring" was deepened with increasing of concentration. One-way analysis results showed that the total length and weight was not significantly different between the experimental groups and the control group (P > 0.05), which means that the SrCl2·6H2O immersion did not affect the growth of young fish. A Chi-square test (P > 0.05) showed there were no significant differences in mortality rate among the different concentration groups. Overall, 32 mg/L SrCl2·6H2O was the best marker concentration. |
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| Keywords: | strontium marking Paralichthys olivaceus otolith SrCl2· 6H2O |
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