| 陈氏刺棘虫感染对洄游型刀鲚肠道微生物群落的影响 |
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| 引用本文： | 姜敏,张希昭,杨彦平,尹登花,代培,应聪萍,刘凯.陈氏刺棘虫感染对洄游型刀鲚肠道微生物群落的影响[J].中国水产科学,2019,26(3):577-585. |
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| 作者姓名： | 姜敏 张希昭 杨彦平 尹登花 代培 应聪萍 刘凯 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院, 上海 201306;2. 中国水产科学研究院淡水渔业研究中心, 农业农村部长江下游渔业资源环境科学观测实验站, 江苏 无锡 214081 |
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| 基金项目： | 农业财政专项“长江下游渔业资源与环境调查”（CJDC-2017-22）；农业部物种资源保护项目（专项捕捞品种资源调查及分析）；长江三峡工程生态与环境监测系统水生生物与渔业环境监测重点站（下游站）监测项目（JJ[2017]-010）. |
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| 摘 要： | 于2017年洄游汛期内采集刀鲚(Coilianasus)样本,解剖后选取6尾感染陈氏刺棘虫(Acanthosentischeni)的刀鲚样本作为感染组,同时选取6尾未感染刀鲚作为对照组,采用16S rDNA高通量测序技术研究陈氏刺棘虫感染对刀鲚肠道微生物群落结构的影响。结果共鉴定出23个细菌门,其中对照组包含22个门,感染组鉴定出18个门,其中疣微菌门(Verrucomicrobia)只在感染组刀鲚中检测到;两组刀鲚肠道中主要的微生物类群均为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和软壁菌门(Tenericutes),且相对丰度总量均高于85%;感染组中厚壁菌门、梭杆菌门(Fusobacteria)和纤维杆菌门(Fibrobacteres)平均含量高于对照组,其余菌门相对丰度含量与对照组相比均呈下降趋势,但两组间微生物组成比例没有显著差异(P0.05)。此外,在已明确的130个科中,厚壁菌门中的梭菌科(Clostridiaceae)、消化链球菌科(Peptostreptococcaceae)、乳杆菌科(Lactobacillaceae)和变形菌门中的肠杆菌科(Enterobacteriaceae)含量高于对照组(P0.05),紫单胞菌科(Porphyromonadaceae)和毛螺菌科(Lachnospiraceae)中的Eisenbergiella丰富度在感染组刀鲚肠道中显著更高(P0.05);在群落多样性水平上,对照组和感染组间Shannon指数、Chao1指数和Simpson指数差异不显著(P0.05)。虽然陈氏刺棘虫感染对肠道微生物的群落结构和物种多样性影响不显著,但是在特定微生物物种的组成上存在显著差异。
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| 关 键 词： | 刀鲚 陈氏刺棘虫 16S rDNA 肠道微生物 |
| 修稿时间： | 2019/5/16 0:00:00 |
Effects of Acanthosentis cheni infection on microbiota composition and diversity in the intestine of Coilia nasus |
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| JIANG Min,ZHANG Xizhao,YANG Yanping,YIN Denghu,DAI Pei,YING Congping,LIU Kai.Effects of Acanthosentis cheni infection on microbiota composition and diversity in the intestine of Coilia nasus[J].Journal of Fishery Sciences of China,2019,26(3):577-585. |
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| Authors: | JIANG Min ZHANG Xizhao YANG Yanping YIN Denghu DAI Pei YING Congping LIU Kai |
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| Institution: | 1. Shanghai Ocean University, Shanghai 201306, China;2. Scientific Observing and Experimental Station of Fishery Resources, Chinese Academy of Fishery Sciences, Wuxi 214081, China |
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| Abstract: | Coilia nasus samples were collected during the migration flood season of 2017. After dissection, six samples of C. nasus infected with Acanthosentis cheni were selected as the infected group, and six samples that were uninfected were selected as the control group to determine the effect of A. cheni infection on the intestinal microbial community structure of C. nasus. A total of twenty-three bacterial phyla were identified. Twenty-two phyla were identified in the control group and eighteen phyla were identified in the infected group, among which Verrucomicrobia was only detected in the infected group. The main bacteria phyla in the intestinal tract of C. nasus were Proteobacteria, Firmicutes, and Tenericutes, with a total relative abundance of more than 85%. The average content of Firmicutes, Fusobacteria, and Fibrobacteres in the infected group was higher than that in the control group, and the relative abundance of other bacteria phyla was lower than that in the control group, but there was no significant difference between the two groups (P > 0.05). Comparison of the relative abundance of bacteria at the family level showed that the proportions of Clostridiaceae, Peptostreptococcaceae, Lactobacillaceae from Firmicutes, and Enterobacteriaceae in the Proteobacteria were higher than those in the control group. Abundance of Peptostreptococcaceae, Lactobacillaceae, and Enterobacteriaceae in the 130 families identified were higher than those in the control group. Porphyromonadaceae and Eisenbergiella were significantly more abundant in the intestinal tract of the infected group (P < 0.05). At the community diversity level, there was no significant difference in Shannon index, Chao1 index, and Simpson index between the two groups (P > 0.05). Although the effects of A. cheni infection on the community structure and species diversity of intestinal microorganisms were not significant, there were significant differences in the composition of specific species. |
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| Keywords: | Coilia nasus Acanthosentis cheni 16S rDNA intestinal microbiota |
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