| 猪轮状病毒GD株VP6基因的克隆及序列分析 |
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| 引用本文： | 田小艳,邓雨修,孙华,苏润环,王东东,宋延华.猪轮状病毒GD株VP6基因的克隆及序列分析[J].中国动物检疫,2010,27(2):33-34,41. |
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| 作者姓名： | 田小艳 邓雨修 孙华 苏润环 王东东 宋延华 |
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| 作者单位： | 广东省温氏集团研究院,广东新兴,527400 |
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| 摘 要： | 参考GenBank中发表的PRV OSU毒株VP6基因核苷酸序列ORF两端保守区序列,设计1对特异引物,以PRV GD株反转录cDNA为模板,通过PCR方法扩增出长约1.4 kb的基因片段,并克隆到pMD18-T载体中进行序列测定。测序结果表明:VP6基因全长1320 bp,含有一个1194bp的开放阅读框,编码397个氨基酸。与国内外已知的11个毒株VP6基因的核苷酸及其推导的氨基酸序列比较,同源性分别为76.5%-95.6%和88.7%-99.2%;核苷酸系统发育进化树结果表明,GD毒株与轮状病毒JL94,CN86,OSU毒株亲缘关系较近,为一个进化群。
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| 关 键 词： | 猪轮状病毒 VP6基因 克隆 序列分析 |
Sequence Analysis of VP6 Gene of Porcine Rotavirus GD Strain |
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| TIAN Xiaoyan,DENG Yuxiu,SUN Hu,SU Runhuan,WANG Dongdong,SONG Yanhua.Sequence Analysis of VP6 Gene of Porcine Rotavirus GD Strain[J].China Journal Of Animal Quarantine,2010,27(2):33-34,41. |
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| Authors: | TIAN Xiaoyan DENG Yuxiu SUN Hu SU Runhuan WANG Dongdong SONG Yanhua |
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| Institution: | (Guangdong Win’s Group Academy,Xinxing Guangdong,527400 China) |
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| Abstract: | A pair of primers derived from the conserved region flanking the ORF of VP6 gene of porcine ro-tavirus(PRV)OSU strain was designed according to the published sequence.A PCR product of approximately 1400bp was amplified from cDNA of RV GD strain.The PCR product was inserted into pMD18-T vector and sequenced.The full-length VP6gene contained 1320bp including a coding region of 1194bp,which encodes a pro-tein of 397 amino acids.The VP6 gene of the RV GD strain shared 76.5%~95.6% homology of necleotide se-quences and 88.7%~99.2% amino acids with those of 11 RV strains.The results indicated that the GD isolate is more similar to JL94、CN86 and OUS in gene type. |
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| Keywords: | porcine rotaviru(PRV) VP6 genome cloning sequence analysis |
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