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| 盐酸克伦特罗单克隆抗体的制备及其特性 | |
| 引用本文： | 王选年,杨艳艳,李青梅,邢广旭,李学伍,肖治军,邓瑞广,柴书军,杨继飞,赵东,康晓迪,张改平.盐酸克伦特罗单克隆抗体的制备及其特性[J].河南农业科学,2002(6):30-33. |
| 作者姓名： | 王选年 杨艳艳 李青梅 邢广旭 李学伍 肖治军 邓瑞广 柴书军 杨继飞 赵东 康晓迪 张改平 |
| 作者单位： | 1. 河南职业技术师范学院,新乡,453003 2. 河南省农业科学院生物技术研究所 3. 河南百奥生物工程有限公司 |
| 基金项目： | 河南省科技攻关重大项目资助 |
| 摘 要： | 以戊二醛法将盐酸克伦特罗 (Clenbuterol,CL)连接到牛血清白蛋白 (BSA)上制备抗原(BSA -CL) ,并以BSA -CL免疫Balb/c小鼠 ,应用杂交瘤技术将免疫鼠脾细胞与NS0细胞融合 ,建立分泌CL单克隆抗体的杂交瘤细胞株。通过对杂交瘤细胞培养上清的检测、鉴定 ,筛选出 1 7株高亲和力的杂交瘤细胞株 ,其中C - 4C9、C - 2D6、C - 4G1和C - 2H6的培养上清ELISA效价为 1∶5 1 2 ,1∶640 ,1∶81 0和 1∶2 5 6;腹水ELISA效价为 5× 1 0 - 6,1 .7× 1 0 - 5,2×1 0 - 6和 3.3× 1 0 - 5。 4株单抗与 β -肾上腺素激动剂沙丁胺醇的交叉反应性为 0 .79% ,与肾上腺素、去甲肾上腺素、异丙肾上腺素及畜禽常用抗生素无交叉反应性。可以作为制备CL快速检测的特异性单克隆抗体应用 |
| 关 键 词： | β-肾上腺素激动剂 盐酸克伦特罗 单克隆抗体 交叉反应 |
| 修稿时间： | 2002年2月24日 |
Preparation and Characteristic of Monoclonal Antibodies for the β-Adrenergic Agonist Clenbuterol Hydrochloride |
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| WANG Xuan nian ,YANG Yan yan ,LI Qing mei ,XING Guang xu ,LI Xue wu ,XIAO Zhi jun ,DENG Rui guang ,CAI Shu jun ,YANG Ji fei ,ZHAO Dong ,KANG Xiao di ,ZHANG Gai ping.Preparation and Characteristic of Monoclonal Antibodies for the β-Adrenergic Agonist Clenbuterol Hydrochloride[J].Journal of Henan Agricultural Sciences,2002(6):30-33. | |
| Authors: | WANG Xuan nian YANG Yan yan LI Qing mei XING Guang xu LI Xue wu XIAO Zhi jun DENG Rui guang CAI Shu jun YANG Ji fei ZHAO Dong KANG Xiao di ZHANG Gai ping |
| Institution: | WANG Xuan nian 1,YANG Yan yan 2,LI Qing mei 2,XING Guang xu 3,LI Xue wu 2,XIAO Zhi jun 2,DENG Rui guang 2,CAI Shu jun 3,YANG Ji fei 2,ZHAO Dong 3,KANG Xiao di 3,ZHANG Gai ping 2 |
| Abstract: | Monoclonal antibodies(mAb) against clenbuterol hydrochloride(CL),a β adrenergic agonist, which was conjugated with Bovine Sera Albumin(BSA), were produced using conventional cell fusion technology.17 high affinity hybridoma lines were screened for specificity to CL by an enzyme-linked immunosorbent assay (ELISA). The titers of the hybridoma supernatants of four clones in these 17 lines were 1∶512 in C 4C9,1∶640 in C 2D6,1∶810 in C 4G1 and 1∶256 in C 2H6,and the ascites titers were 5×10 -6 ,1.7×10 -5 ,2×10 -6 ,and 3.3×10 -5 ,respectively.The mAb generally had 0.79% cross reactivity towards Salbuterol, and showed no cross reactivity towards Epinephrine, Norepinephrine, Isoprenaline, and antibiotics that used in animal feed. |
| Keywords: | Adrenergic Agonist Clenbuterol hydrochloride Monoclonal antibody Cross reactivity |
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