| 农杆菌介导的果树转基因研究进展 |
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| 引用本文： | 刘松瑜,谢深喜,陶爱群,周亚洲,周 力,沈程清.农杆菌介导的果树转基因研究进展[J].中国农学通报,2009,25(9):179-183. |
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| 作者姓名： | 刘松瑜 谢深喜 陶爱群 周亚洲 周 力 沈程清 |
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| 作者单位： | 湖南农业大学园艺园林学院,长沙,410128 |
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| 摘 要： | 农杆菌介导法在果树遗传改良中有广阔的应用前景，农杆菌介导法的受体系统有原生质体﹑愈伤组织﹑直接分化芽和胚状体受体系统等；影响农杆菌转化频率的因素有侵染力,抗生素,基因型等；常用检测方法有GUS活性检测，PCR技术，杂交检测，免疫学检测。本文从农杆菌介导法在果树中的各种转化受体系统、影响果树基因转化的主要因素和转化植株的检测等方面进行综述，为不同果树建立高效的基因转化体系提供参考。
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| 关 键 词： | 受体系统 农杆菌 侵染力 检测 |
| 收稿时间： | 2009-01-09 |
| 修稿时间： | 2009-02-21 |
Research Progress of Gene Transformation in fruit tree using Agrobacterium tumefaciens |
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| Liu Songyu,Xie Shenxi,Tao Aiqun,Zhou Yazhou,Zhou Li,Shen Chengqing.Research Progress of Gene Transformation in fruit tree using Agrobacterium tumefaciens[J].Chinese Agricultural Science Bulletin,2009,25(9):179-183. |
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| Authors: | Liu Songyu Xie Shenxi Tao Aiqun Zhou Yazhou Zhou Li Shen Chengqing |
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| Institution: | (Horticulture and Landscape College, Hunan Agricultural University, Changsha 410128) |
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| Abstract: | Agrobacterium-mediated method has broad application prospects in the genetic improvement of fruit trees. The Agrobacterium-mediated receptor system has protoplast receptor systems, callus receptor systems, direct bud differentiation receptor system and embryoid Body systems.The factors of affecting the frequency of Agrobacterium-mediated transformation include infection, antibiotics, genotype.Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection. This article is reviewed on the Agrobacterium-mediated in a variety receptor system of fruit trees,transforming ways, main factors affecting transfermation of fruit genes and the detections and so on, and provide reference for different fruit trees to establish highly efficient gene transfer systems. |
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| Keywords: | receptor system Agrobacterium infective ability detection |
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