| BSA对牛结核抗体ELISA检测的影响 |
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| 引用本文： | 吴庆儒,郭定宗,张桂荣,李文波.BSA对牛结核抗体ELISA检测的影响[J].动物医学进展,2007,28(5):5-9. |
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| 作者姓名： | 吴庆儒 郭定宗 张桂荣 李文波 |
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| 作者单位： | 华中农业大学动物医学院,湖北武汉,430070 |
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| 摘 要： | 分别研究了封闭液、血清稀释液及酶标二抗稀释液中BSA成分对牛结核抗体ELISA检测的影响.结果显示,酶标二抗稀释液中BSA的存在使ELISA检测值普遍偏低,且BSA的含量越高,检测值越低;为了提高ELISA检测值,使其达到理想检测效果,必须提高酶标二抗的使用浓度.BSA中可能存在的牛血清抗体杂质对酶标二抗起到了"中和"作用,降低了酶标二抗与目标抗体结合的实际浓度,使检测结果普遍偏低.
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| 关 键 词： | 牛血清白蛋白 酶联免疫吸附试验 酶标二抗 |
| 文章编号： | 1007-5038（2007）05-0005-05 |
| 收稿时间： | 2007-02-04 |
| 修稿时间： | 2007年2月4日 |
The Effects of BSA on Detecting of Mycobacterium bovis Antibody by ELISA |
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| WU Qing-ru,GUO Ding-zong,ZHANG Gui-rong,LI Wen-bo.The Effects of BSA on Detecting of Mycobacterium bovis Antibody by ELISA[J].Progress In Veterinary Medicine,2007,28(5):5-9. |
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| Authors: | WU Qing-ru GUO Ding-zong ZHANG Gui-rong LI Wen-bo |
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| Abstract: | In this study,bovine serum albumin(BSA) was used as components of blocking buffer,diluents for serum and enzyme-labeled secondary antibody,respectively.And the effects of BSA on ELISA which was used to detect antibodies of Mycobacterium bovis was discussed.The component of BSA in diluent for enzyme-labeled secondary antibody was found to significantly lower the read of OD630 in ELISA test,and with the increasing of BSA in diluent for enzyme-labeled secondary antibody,the read of OD630 in ELISA test fell.In order to heighten the read of ELISA test and reach an ideal detection effects,we had to heighten concentration enzyme-labeled secondary antibody.Based on the above research,we got that there was some antibody of bovine serum in BSA.The enzyme-labeled secondary antibody was counteracted by these antibody,and the concentration of which was reduced when it reacted with target antibody.Then read of ELISA test was lowered. |
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| Keywords: | BSA ELISA enzyme-labeled secondary antibody |
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