Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
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| Authors: | Martella Vito Elia Gabriella Lucente Maria Stella Decaro Nicola Lorusso Eleonora Banyai Krisztian Blixenkrone-Møller Merete Lan Nguyen Thi Yamaguchi Ryoji Cirone Francesco Carmichael Leland Eugene Buonavoglia Canio |
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| Institution: | Department of Animal Health and Well-being, University of Bari, Valenzano, Bari, Italy. v.martella@veterinaria.uniba.it |
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| Abstract: | CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-na?ve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease. |
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