| 鸭出血性卵巢炎的初步研究 |
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| 引用本文： | 曹贞贞,张存,黄瑜,刁有祥,叶伟成,刘月焕,韩婧文,马国明,张冬冬,许丰,王丹,姜甜甜,袁媛,谢小雨,高绪慧,唐熠,施少华,万春和,张晨,何玢,杨梦婕,陆新浩,张冰,张国中,马学军,张大丙.鸭出血性卵巢炎的初步研究[J].中国兽医杂志,2010,46(12). |
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| 作者姓名： | 曹贞贞 张存 黄瑜 刁有祥 叶伟成 刘月焕 韩婧文 马国明 张冬冬 许丰 王丹 姜甜甜 袁媛 谢小雨 高绪慧 唐熠 施少华 万春和 张晨 何玢 杨梦婕 陆新浩 张冰 张国中 马学军 张大丙 |
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| 作者单位： | 中国农业大学动物医学院农业部人畜共患病重点开放实验室;浙江省农业科学院畜牧兽医研究所;福建省农业科学院畜牧兽医研究所;山东农业大学动物科技学院;北京市农林科学院畜牧兽医研究所;北京农学院动物科技系;中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室;浙江省余姚畜禽防治研究所; |
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| 基金项目： | 现代农业产业技术体系建设专项资金，传染病重大专项 |
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| 摘 要： | 2010年6月以来,我国部分地区所饲养的种鸭和蛋鸭发生一种疾病,以突发产蛋急剧下降为特点,根据病变,暂将该病称为鸭出血性卵巢炎(duck hemorrhagic ovaritis,DHO)。从不同地区的发病鸭群采集34份样品,取15份样品进行病毒分离,获得10个鸡胚分离物和5个鸭胚分离物。RT-PCR检测结果表明,15个分离物均为禽流感阴性,14个分离物为新城疫阴性。选分离株YY5株进行了电镜观察和PCR排查,结果表明,该毒株的毒粒直径为40~50 nm,呈鸭瘟病毒、水禽细小病毒、鸭呼肠孤病毒、鸭甲肝病毒、鸭星状病毒、鸭圆环病毒、鸭冠状病毒、鸡传染性支气管炎病毒PCR阴性,但为黄病毒RT-PCR阳性。基于黄病毒NS1序列合成引物,用RT-PCR检测15株病毒分离物和其余19份临床样品,黄病毒阳性率为82.4%。用YY5株的221-nt NS1序列进行分析的结果显示,YY5株与黄病毒科黄病毒属恩塔亚病毒群(Ntaya virus group)的巴格扎病毒(Bagaza virus)具有相近的遗传进化关系,但遗传距离介于病毒种的水平。用一株鸭胚分离株感染91日龄临近开产的麻鸭和232日龄的产蛋麻鸭,可复制出卵泡膜出血的病变。结果表明,从DHO自然病例分离到一种新的黄病毒,暂称为鸭黄病毒(duck flavivirus,DFV),DFV可能与DHO有关。
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| 关 键 词： | 鸭出血性卵巢炎 鸭黄病毒 种鸭 蛋鸭 产蛋下降 |
Preliminary studies on duck hemorrhagic ovaritis |
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| CAO Zhen-zhen,ZHANG Cun,HUANG Yu,DIAO You-xiang,YE Wei-cheng,LIU Yue-huan,HAN Jing-wen,MA Guo-ming,ZHANG Dong-dong,XU Feng,WANG Dan,JIANG Tian-tian,YUAN Yuan,XIE Xiao-yu,GAO Xu-hui,TANG Yi,SHI Shao-hua,WAN Chun-he,ZHANG Chen,HE Bin,YANG Meng-jie,LU Xin-hao,ZHANG Bin,ZHANG Guo-zhong,MA Xue-jun,ZHANG Da-bing.Preliminary studies on duck hemorrhagic ovaritis[J].Chinese Journal of Veterinary Medicine,2010,46(12). |
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| Authors: | CAO Zhen-zhen ZHANG Cun HUANG Yu DIAO You-xiang YE Wei-cheng LIU Yue-huan HAN Jing-wen MA Guo-ming ZHANG Dong-dong XU Feng WANG Dan JIANG Tian-tian YUAN Yuan XIE Xiao-yu GAO Xu-hui TANG Yi SHI Shao-hua WAN Chun-he ZHANG Chen HE Bin YANG Meng-jie LU Xin-hao ZHANG Bin ZHANG Guo-zhong MA Xue-jun ZHANG Da-bing |
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| Institution: | CAO Zhen-zhen1,ZHANG Cun2,HUANG Yu3,DIAO You-xiang4,YE Wei-cheng2,LIU Yue-huan5,HAN Jing-wen5,MA Guo-ming1,ZHANG Dong-dong1,XU Feng6,WANG Dan1,JIANG Tian-tian1,YUAN Yuan1,XIE Xiao-yu1,GAO Xu-hui4,TANG Yi4,SHI Shao-hua3,WAN Chun-he3,ZHANG Chen7,HE Bin7,YANG Meng-jie7,LU Xin-hao8,ZHANG Bin1,ZHANG Guo-zhong1,MA Xue-jun7,ZHANG Da-bing1(1.Key Laboratory of Zoonosis of Ministry of Agriculture,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.Institute of Animal Husbandry and Vete... |
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| Abstract: | From June through November 2010,a disease characterized by a sudden onset was observed in many egg-laying and breeder duck farms in China.Based on the pathological changes,the disease was tentatively called duck hemorrhagic ovaritis(DHO).Thirty-four samples were collected from the diseased ducks in different regions and 15 of the samples were subjected to virus isolation by using SPF chicken and duck embryos.Fifteen isolates were obtained.RT-PCR results showed that all the 15 isolates were negative for avia... |
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| Keywords: | duck hemorrhagic ovaritis duck flavivirus breeder ducks laying ducks egg drop |
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