| 发病罗非鱼苗沃氏葡萄球菌的分离与鉴定 |
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| 引用本文： | 李晓陀,陈秦,廖承红,郭桂英,李祥,贾晓琳,郑继平,俞集楠.发病罗非鱼苗沃氏葡萄球菌的分离与鉴定[J].热带生物学报,2013,4(1):7-10. |
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| 作者姓名： | 李晓陀 陈秦 廖承红 郭桂英 李祥 贾晓琳 郑继平 俞集楠 |
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| 作者单位： | 1. 海南大学热带生物资源教育部重点实验室,海南海口,570228
2. 海南大学热带生物资源教育部重点实验室,海南海口570228;海南大学教务处,海南海口570228 |
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| 基金项目： | 海南大学校青年基金（qnjj1147,qnjj1229）; 海南省自然科学基金项目（313043） |
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| 摘 要： | 为检测发病罗非鱼苗中可能存在的潜在病原感染,保证投放鱼苗的健康安全,对海口市发病罗非鱼苗进行了菌种分离.结果发现,在发病鱼苗中存在葡萄球菌,经16S rRNA基因序列测定,显示该菌与沃氏葡萄球菌和巴斯德葡萄球菌高度相似,后经超氧(化)物歧化酶sodA基因PCR分析,确认为沃氏葡萄球菌.
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| 关 键 词： | 罗非鱼 沃氏葡萄球菌 16S rRNA sodA |
Isolation and Identification of Staphylococci warneri
from Diseased Tilapia Fry |
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| LI Xiaotuo,CHEN Qin,LIAO Chenghong,GUO Guiying,LI Xiang,JIA Xiaolin,ZHENG Jiping,YU Jinan.Isolation and Identification of Staphylococci warneri
from Diseased Tilapia Fry[J].Journal of Tropical Biology,2013,4(1):7-10. |
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| Authors: | LI Xiaotuo CHEN Qin LIAO Chenghong GUO Guiying LI Xiang JIA Xiaolin ZHENG Jiping YU Jinan |
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| Institution: | 1 ( 1. Key Laboratory of Tropical Biological Resources,Ministry of Education; 2. Academic Affairs Office,Hainan University,Haikou 570228,China) |
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| Abstract: | Strains of Staphylococci,the largest Gram positive stains,were isolated by direct plating and Gram stain from the samples of livers and gallbladders from the diseased Tilapia fry collected in Haikou. Analysis of 16S rRNA gene sequence showed that these isolates were highly homologous with Staphylococcus pasteuri and Staphylococcus warneri,but further identified to be Staphylococcus warneri by PCR amplification of the sodA gene for superoxide dismutase. |
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| Keywords: | tilapia Staphylococcus warneri 16S rRNA sodA gene |
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