| 蟒蛇铜绿假单胞菌的分离鉴定及抗药性分析 |
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| 引用本文： | 曾纪锋,林杰材,李笑春,郑继平,廖世庄.蟒蛇铜绿假单胞菌的分离鉴定及抗药性分析[J].热带生物学报,2013,4(2):173-176. |
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| 作者姓名： | 曾纪锋 林杰材 李笑春 郑继平 廖世庄 |
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| 作者单位： | [1]海南大学农学院,海南海口570228 [2]海南东盛弘蟒蛇科技有限公司,海南文昌571346 |
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| 基金项目： | 海南省自然科学基金项目（309003） |
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| 摘 要： | 为了确认死于急性支气管炎、肺炎的蟒蛇致病病原,对死亡蟒蛇的肝、肺组织及心血进行病原分离培养,获得1株分离菌株,并对其进行形态学、培养特性、生化特性测定及动物致病试验。结果表明,分离菌株为铜绿假单胞菌,是本病致病病原;对分离菌株进行24种常用抗菌药物的药敏试验,结果表明,该菌株对氟苯尼考、妥布霉素、氯霉素和环丙沙星敏感,对红霉素、氟哌酸、氨苄青霉素、头孢拉定、新霉素、复方新诺明、卡那霉素、罗红霉素、丁胺卡那霉素、氧氟沙星、阿莫西林、庆大霉素、四环素等多种抗菌药物产生耐药,且呈多重耐药性。
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| 关 键 词： | 铜绿假单胞菌 分离鉴定 抗药性 蟒蛇 |
Identification of a Strain of Pseudomonas Aeruginosa Isolated from Python and Its Tolerance to Antibiotics |
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| ZENG Jifeng,LIN Jiecai,LI Xiaochun,ZHENG Jiping,LIAO Shizhuang.Identification of a Strain of Pseudomonas Aeruginosa Isolated from Python and Its Tolerance to Antibiotics[J].Journal of Tropical Biology,2013,4(2):173-176. |
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| Authors: | ZENG Jifeng LIN Jiecai LI Xiaochun ZHENG Jiping LIAO Shizhuang |
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| Institution: | 1. College of Agronomy,Hainan University,Haikou 570228,China; 2. Hainan Dongshenghong Python Co. Ltd. ,Wenchang 571346,China) |
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| Abstract: | Based on the colonial morphology,Gram＇s staining,bacteria growth requirements,biochemical properties,and animal inoculation tests,a Pseudomonas aeruginosa strain was isolated and identified from the tissues of the python （ Python molurus） dead of pneumonia and acute bronchitis. The strain was tested with 24 different antibiotics to determine its tolerance against the antibiotics. The results showed that the strain was highly sensitive to florfenicol,tobramycine,chloromycine and ciprofloxacin,but tolerant to erythromycin,norfloxacin,ampicillin,cefradine,novomycin,selectrin,kanamycin,roxithromycin,amikacin,ofloxacin,amoxicillin,cidomycin,tetracycline,etc,showing its multi-tolerance to antibiotics. |
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| Keywords: | Pseudomonas aeruginosa isolation and identification tolerance Python molurus |
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