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| PRRSV GP5基因的原核表达及其免疫性分析 | |
| 引用本文： | 扈鸿霞,方维焕,刘建华,冉多良.PRRSV GP5基因的原核表达及其免疫性分析[J].动物医学进展,2008,29(5):32-35. |
| 作者姓名： | 扈鸿霞 方维焕 刘建华 冉多良 |
| 作者单位： | 1. 新疆农业大学动物医学学院,新疆乌鲁木齐,830052;浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州,310029 2. 浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州,310029 3. 新疆农业大学动物医学学院,新疆乌鲁木齐,830052 |
| 摘 要： | 猪繁殖与呼吸综合征病毒(PRRSV)的糖蛋白GP5含有中和表位,是检测用抗原和亚单位疫苗的首选蛋白。本研究将糖蛋白GP5基因截短后,克隆至表达载体pET-30a,转化大肠埃希菌,经IPTG诱导后,SDS-PAGE电泳显示重组蛋白以包涵体形式得到了表达,每升重组菌可纯化得到目的蛋白87.5mg。Western blot和间接酶联免疫吸附试验分析表明,该蛋白与PRRSV阳性血清具有良好免疫反应性。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 结构蛋白GP5 免疫反应性 |
Prokaryotic Expression of GP5 of PRRSV and Analysis of Its Immunoreactivity |
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| HU Hong-xia,FANG Wei-huan,LIU Jian-hua,RAN Duo-liang.Prokaryotic Expression of GP5 of PRRSV and Analysis of Its Immunoreactivity[J].Progress In Veterinary Medicine,2008,29(5):32-35. | |
| Authors: | HU Hong-xia FANG Wei-huan LIU Jian-hua RAN Duo-liang |
| Abstract: | The envelope protein GP5 of porcine reproductive and respiratory syndrome virus contains neutralizing epitope,and thus is the primary target as a detective antigen or a subunit vaccine.In this study,the gene fragment encoding truncated GP5 protein was cloned into the expressive vector pET-30a,and the recombinant protein was efficiently expressed in E.coli Rosetta in the form of inclusion bodies after induction with IPTG in high yield of 87.5 mg purified protein per liter of culture.Western blot and indirect ELISA analyses showed the recombinant protein had good immunoreactivity with the reference positive serum. |
| Keywords: | PRRSV structural protein GP5 immunoactivity |
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