| C/N调控和生态基对草鱼生长性能、水质及微生物活动的影响 |
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| 引用本文： | 王金林,郁二蒙,王广军,余德光,谢骏,李志斐,夏耘,魏南.C/N调控和生态基对草鱼生长性能、水质及微生物活动的影响[J].中国农业大学学报,2016,21(3):75-88. |
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| 作者姓名： | 王金林 郁二蒙 王广军 余德光 谢骏 李志斐 夏耘 魏南 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;上海海洋大学水产与生命学院, 上海 201306;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380;中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室, 广州510380 |
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| 基金项目： | 国家现代农业产业技术体系建设专项(No.CARS-46-17); 农业部公益性行业科研专项(201203083) |
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| 摘 要： | 为研究C/N调控和生态基对草鱼生长性能、水质及微生物活动的影响,利用PCR-DGGE技术对不同C/N条件下草鱼养殖池水体及生态基细菌群落结构的动态变化进行研究,并监测养殖水质指标和草鱼生长状况。在生态基系统中,对照组投喂基础饲料,试验组在基础饲料上添加葡萄糖,控制C/N分别为15∶1(CN15)、20∶1(CN20)和25∶1(CN25)。结果显示:CN20处理组中,草鱼增重率及特定生长率均显著高于其他组(P0.05),饵料系数显著低于其他组(P0.05)。CN25处理组中,溶氧、硝酸态氮、亚硝酸态氮水平均显著低于其他组(P0.05);CN20和CN25处理组中,COD及BOD含量显著高于其他组(P0.05)。同时,生态基中的细菌总量随着C/N的提高逐渐增加,最高值为5.57×107 cells/g。PCR-DGGE结果显示:随着C/N提高,对照组、CN15、CN20和CN25处理组的水体细菌群落组成与水源水体的相似性分别为37%、32%、26%和22%,该4个处理组的生态基细菌群落组成与养殖水体的相似性分别为59%、58%、55%和52%。红细菌(Rhodobacter blasticus)、绿弯菌(Chloroflexi)是各处理组生态基中的共有细菌,并且它们为对照组和CN15组养殖水体的特有菌;拟杆菌(Bacteroidetes)是CN20组养殖水体及生态基中特有优势细菌。结果表明,在生态基系统中,不同C/N影响水体细菌群落向生态基的定居迁移;C/N为20∶1与生态基结合使用可显著促进草鱼生长、提高养殖产量。
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| 关 键 词： | 草鱼 生态基 C/N 细菌群落结构 |
| 收稿时间： | 2015/5/29 0:00:00 |
Effect of C/N ratio and substrate integration on water quality, microbial dynamics and growth of Ctenopharyngodon idellus |
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| WANG Jin-lin,YU Er-meng,WANG Guang-jun,YU De-guang,XIE Jun,LI Zhi-fei,XIA Yun and WEI Nan.Effect of C/N ratio and substrate integration on water quality, microbial dynamics and growth of Ctenopharyngodon idellus[J].Journal of China Agricultural University,2016,21(3):75-88. |
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| Authors: | WANG Jin-lin YU Er-meng WANG Guang-jun YU De-guang XIE Jun LI Zhi-fei XIA Yun and WEI Nan |
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| Institution: | Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China;Pearl River Fishery Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China |
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| Abstract: | To investigate the effect of C/N ratio and substrate integration on water quality, microbial dynamics and growth of Ctenopharyngodon idellus, bacteria community composition of substrate and water bacterial community composition at four C/N ratios was investigated by using PCR-denaturing gradient gel electrophoresis (DGGE) technique.The aquaculture water quality, growth performance of C.idellus were also investigated.An artificially formulated and prepared feed containing 30% crude protein was applied to all ponds.In order to raise the C/N ratio of aquaculture water to 15:1 (CN15), 20:1 (CN20) and 25:1 (CN25), glucose was applied separately as a source of carbohydrate in addition to artificial feed.The results showed that weight gain and specific growth rate of grass carp in CN20 treatment group were significantly higher (P<0.05) than other groups, feed conversion ratio in CN20 group was lower than other groups (P<0.05).Dissolved oxygen, ammonia and nitrite concentration in CN25 group were significantly lower (P<0.05) than other groups, COD and BOD in CN25 group was higher than other groups (P<0.05).The amount of bacteria in substrate gradually increased with the increasing of C/N ratio, and peaked at 5.57×107 cells/g.With the increasing of C/N ratio, bacterial community composition similarity of aquaculture water in the control group andCN15, CN20, and CN25 treatment group with that of source water was 37%, 32%, 26% and 22% respectively;Bacterial community composition similarity between substrate and water was 59%, 58%, 55% and 52% respectively.Rhodobacter blasticus and Chloroflexi, the common bacteria in substrates, existed only in water of control group and CN15 treatment group.Bacteroidetes was dominant bacteria in both water and substrate of CN20 treatment group.The results demonstrated that C/N ratio affects bacterial migration from water to substrate.The C/N ratio of 20:1 and substrate can significantly promote the growth of C.idellus and improve aquaculture production.These results will provide a scientific basis for the further application of substrate in the actual production of C.idellus. |
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| Keywords: | Ctenopharyngodon idellus substrate carbon and nitrogen ratio bacterial community composition |
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