| 艾氏蛇鳗线粒体基因组全序列结构分析和系统发育关系探讨 |
| |
| 引用本文： | 宁子君,刘玉萍,张书飞,高天翔,杨天燕.艾氏蛇鳗线粒体基因组全序列结构分析和系统发育关系探讨[J].中国水产科学,2022,29(9):1264-1276. |
| |
| 作者姓名： | 宁子君 刘玉萍 张书飞 高天翔 杨天燕 |
| |
| 作者单位： | 浙江海洋大学水产学院, 浙江 舟山 316022;中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 广东 广州 510300 |
| |
| 基金项目： | 国家自然科学基金项目(41776171, 32100405)； 广东省渔业生态环境重点实验室开放基金项目(FEEL-2021-7)； 浙江省教育厅一般项目(Y202148225). |
| |
| 摘 要： | 本研究采用高通量测序技术获得了艾氏蛇鳗(Ophichthus evermanni)线粒体基因组全序列, 并对其结构和特征进行了分析。结果表明, 艾氏蛇鳗线粒体基因组全长 17759 bp, 包含了 13 个蛋白编码基因(PCGs)、22 个转运 RNA 基因(tRNA)、2 个核糖体 RNA 基因(rRNA)、2 个控制区(D-loop)和 1 个轻链复制起始区(OL)。线粒体 DNA 全序列的碱基组成分别为 A (31.27%)、G (16.19%)、C (26.22%)和 T (26.32%), 其中 A+T 含量(57.59%)大于 G+C 含量 (42.41%), 呈现出明显的 A+T 偏好性。与大多数硬骨鱼类不同, 艾氏蛇鳗线粒体基因组中发生了基因重排现象, ND6 基因和 tRNA-Glu 移到了 tRNA-Thr 和 tRNA-Pro 之间, 且 ND6 基因上游还存在另一个高度同源的 D-loop 区。 tRNA-Gln (Q)、tRNA-Ala (A)、tRNA-Asn (N)、tRNA-Cys (C)、tRNA-Tyr (Y)、tRNA-SerUCA (S1)、tRNA-Glu (E)、 tRNA-Pro (P)和 ND6 9 个基因位于 L 链, 其余基因均位于 H 链。除 tRNA-Ser (AGC)外, 其余 21 个 tRNA 均为典型的三叶草二级结构。分别采用邻接法和最大似然法, 基于 12 个蛋白编码基因(ND6 除外)构建了蛇鳗科鱼类系统发育关系树。结果显示艾氏蛇鳗与短尾蛇鳗(O. brevicaudatus)和食蟹豆齿蛇鳗(Pisodonophis cancrivorus)的亲缘关系较近, 蛇鳗属是蛇鳗科鱼类中分化较晚的一个类群。研究结果丰富了蛇鳗科鱼类线粒体基因组数据库, 也为该类群鱼类的系统分类研究提供了参考资料。
|
| 关 键 词： | 艾氏蛇鳗 高通量测序 线粒体基因组 系统发育分析 |
Structural analysis and phylogenetic relationships of Ophichthus evermanni based on the complete mitochondrial genome sequence |
| |
| NING Zijun,LIU Yuping,ZHANG Shufei,GAO Tianxiang,YANG Tianyan.Structural analysis and phylogenetic relationships of Ophichthus evermanni based on the complete mitochondrial genome sequence[J].Journal of Fishery Sciences of China,2022,29(9):1264-1276. |
| |
| Authors: | NING Zijun LIU Yuping ZHANG Shufei GAO Tianxiang YANG Tianyan |
| |
| Abstract: | In this study, the complete mitochondrial genome of Ophichthus evermanni was obtained by high-throughput sequencing. The results of structure and characteristics analysis showed that the total length of the complete mitochondrial DNA was 17759 bp, including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNA), 2 ribosomal RNAs (rRNA), 2 control regions (D-loop), and 1 origin of the L-strand replication region (OL). The base composition of the complete mitogenome of O. evermanni was A (31.27%), G (16.19%), C (26.22%), and T (26.32%). The A+T content (57.59%) was greater than the G+C content (42.41%), showing an obvious A+T bias. Unlike other typical teleosts, the gene arrangement was identified in the mitogenome of O. evermanni. The ND6 gene and the conjoint tRNA-Glu were translocated between tRNA-Thr and tRNA-Pro, and another highly homologous D-loop region was located upstream of the ND6 gene. The tRNA-Gln (Q), tRNA-Ala (A), tRNA-Asn (N), tRNA-Cys (C), tRNA-Tyr (Y), tRNA-SerUCA (S1), tRNA-Glu (E), tRNA-Pro (P), and ND6 were located in the L-strand, whereas the remainder were located in the H-strand. Except for tRNA-Ser (AGC), the remaining 21 tRNAs could fold into the typical cloverleaf secondary structure. The phylogenetic relationships of Ophichthidae fishes were constructed based on 12 protein-coding genes (excluding ND6) using the neighbor-joining and Bayesian methods. The results suggested that O. evermanni was closely related to O. brevicaudatus and Pisodonophis cancrivorus, and genetic divergence occurred in the genus Ophichthus, which was relatively late among the family Ophichthidae. The results will supplement the mitochondrial genome database of the family Ophichthidae and also provide a reference for the taxonomic study of snake eels. |
| |
| Keywords: | |
|
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
