| Myo5a特异性片段原核表达重组质粒的构建 |
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| 引用本文： | 游荷花,沈敬华.Myo5a特异性片段原核表达重组质粒的构建[J].山西农业大学学报(自然科学版),2012,32(5). |
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| 作者姓名： | 游荷花 沈敬华 |
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| 作者单位： | 1. 山西医科大学汾阳学院,山西汾阳032200;内蒙古医学院,内蒙古呼和浩特010000
2. 内蒙古医学院,内蒙古呼和浩特,010000 |
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| 摘 要： | 为了构建Myo5a特异性片段的原核表达重组质粒PGEX-4T-3/Myo5a,采用PCR扩增出Myo5a基因特异性片段的蛋白编码序列,经BamH I和Xho I双酶切后,将片段插入原核表达载体pGEX-4T-3,构建重组子PGEX-4T-3/Myo5a。最后重组质粒PGEX-4T-3/Myo5a经鉴定完全正确。
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| 关 键 词： | Myo5a 原核表达 重组质粒 |
Construction of Prokaryotic Expression Recombinant Plasmid of Specific Fragment Human Myo5a |
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| You Hehua,Shen Jinghua.Construction of Prokaryotic Expression Recombinant Plasmid of Specific Fragment Human Myo5a[J].Journal of Shanxi Agricultural University,2012,32(5). |
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| Authors: | You Hehua Shen Jinghua |
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| Abstract: | To construct prokaryotic expression recombinant plasmid PGEX-4T-3/Myo5a of Myo5a gene,PCR was used to obtain 131bp coding region of Myo5a.The Myo5a gene was digested by restrictive enzyme Bam HI and Xho I.The gene was inserted into the prokaryotic GST fusion protein expression plasmid pGEX-4T-3,to form PGEX-4T-3/Myo5a.The recombinant plasmid PGEX-4T-3/Myo5a was identified to be correct. |
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| Keywords: | Myo5a Prokaryotic expression Recombinant plasmid |
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