| 大蒜干腐病病原菌的分离鉴定 |
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| 引用本文： | 李旭双,陈典,梁誉,王勇.大蒜干腐病病原菌的分离鉴定[J].中国蔬菜,2012,1(20):88-93. |
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| 作者姓名： | 李旭双 陈典 梁誉 王勇 |
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| 作者单位： | 东北农业大学园艺学院，黑龙江哈尔滨 150030 |
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| 基金项目： | 公益性行业(农业)科研专项(200903018-6) |
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| 摘 要： | 以感病的大蒜品系Z-1001鳞茎为材料，经病原菌分离与单孢纯化，获得F、J、H、G 4种纯化菌株。依据病原菌致病性测定，证实F菌株、J菌株为大蒜干腐病主要致病菌株。采用病原菌形态学观察并结合rDNA ITS序列分析，鉴定F菌株为尖孢镰刀菌（Fusarium oxysporum Schlecht），J菌株为层生镰刀菌〔Fusarium proliferatum（Matsush.）Nirenberg〕，说明尖孢镰刀菌（F.?oxysporum Schlecht）和层生镰刀菌〔F.?proliferatum（Matsush.）Nirenberg〕是大蒜干腐病的主要致病菌。
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| 关 键 词： | 大蒜干腐病 尖孢镰刀菌 层生镰刀菌 形态学 rDNA ITS序列 |
Isolation and Identification of Garlic Dry Rot Disease Pathogen |
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| LI Xu-shuang
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CHEN Dian
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LIANG Yu
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WANG Yong.Isolation and Identification of Garlic Dry Rot Disease Pathogen[J].China Vegetables,2012,1(20):88-93. |
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| Authors: | LI Xu-shuang CHEN Dian LIANG Yu WANG Yong |
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| Institution: | College of Horticulture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
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| Abstract: | In order to study the pathogens of garlic dry rot disease,this study used the susceptible garlic(Allium sativum L.)strain Z-1001 for pathogen isolation,single-spore purification and pathogenicity determination.The results revealed that the F strain and J strain were main garlic dry rot pathogenic strains.The results of pathogen morphology observation and rDNA ITS sequence analysis showed that F strain was Fusarium oxysporum Schlecht,while J strain was Fusarium proliferatum(Matsush.)Nirenberg,which in turn revealed that F. oxysporum Schlecht and F. proliferatum(Matsush.)Nirenberg were the main pathogens of garlic dry rot disease. |
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| Keywords: | Garlic dry rot disease Fusarium oxysporum Schlecht Fusarium proliferatum (Matsush )Nirenberg Morphology rDNA ITS sequence |
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