| 北京鸭MC4R基因的克隆及其组织表达的差异 |
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| 引用本文： | 王 婕,刘小林,侯水生,吴 艳,范宏静.北京鸭MC4R基因的克隆及其组织表达的差异[J].西北农业学报,2011,20(1):29-34. |
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| 作者姓名： | 王 婕 刘小林 侯水生 吴 艳 范宏静 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100
2. 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 国家“十一五”支撑计划项目(2006BAD14B06) |
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| 摘 要： | 黑素皮质素受体-4(melanocortin receptor-4,MC4R)是黑素皮质素受体家族5个亚型(MCR1-5)之一,在控制食欲、体质量、能量平衡中有重要作用。本研究采用RT-PCR技术从北京鸭脑组织中克隆出鸭MC4R基因的编码区序列,分析其基因结构并进行功能预测,利用实时荧光定量进行组织差异表达研究。结果表明,鸭MC4R基因编码区全长996 bp,编码332个氨基酸,包括起始密码子ATG和终止密码子TAG;与鹅、鸡、小鼠、人的相似性分别为97%、95%、78%、80%;推导的氨基酸序列显示,鸭MC4R蛋白具有G蛋白耦联受体家族结构域;实时定量结果表明,MC4R基因在北京鸭各组织相对表达水平存在差异,其中在脑组织中表达最高,脾脏、心脏、腿肌、腹脂、肾脏次之,而在肝脏和肺脏中表达量最低。本试验为进一步研究鸭MC4R基因的生物学功能奠定了基础。
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| 关 键 词： | MC4R 克隆 序列分析 实时定量 差异表达 |
Cloning and Expression Pattern ofMC4R Gene in Beijing Duck |
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| WANG Jie,LIU Xiaolin,HOU Shuisheng,WU Yan and FAN Hongjing.Cloning and Expression Pattern ofMC4R Gene in Beijing Duck[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(1):29-34. |
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| Authors: | WANG Jie LIU Xiaolin HOU Shuisheng WU Yan and FAN Hongjing |
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| Institution: | WANG Jie1,LIU Xiaolin1,HOU Shuisheng2,WU Yan1 and FAN Hongjing1(1.College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling Shaanxi 712100,China,2.State Key Laboratory of Animal Nutrition Science,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | Melanocortin receptor-4(MC4R),one member of melanocortin receptors(MC1-5R) family,plays an important role in food intake,body weight and energy balance.The coding region sequence of MC4R gene was cloned from total RNA of brain in Beijing duck by RT-PCR.The structure and function of MC4R gene were predicted,and the expression pattern was studied by real-time quantity PCR.The results showed that sequence of duck MC4R gene was 996 bp,and encoded 332 amino acids,including start codon ATG and stop codon TAG.Homo... |
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| Keywords: | MC4R Clone Sequence analysis Real-time quantitative PCR Expression pattern |
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