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雾培与液培对黄瓜根系氮代谢相关酶活性的影响
引用本文:刘广晶,孙周平,包美丽,赵 乐,薛东东.雾培与液培对黄瓜根系氮代谢相关酶活性的影响[J].西北农业学报,2011,20(3):132-137.
作者姓名:刘广晶  孙周平  包美丽  赵 乐  薛东东
作者单位:设施园艺省部共建教育部重点实验室,沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳,110866
基金项目:辽宁省教育厅创新团队项目(2007T159)
摘    要:采用雾培和液培(深液流)栽培系统,研究了不同无土栽培方式对津绿‘21-10’和迷你黄瓜‘22-405’根系氮代谢的影响。结果表明,在整个生育期,雾培和液培黄瓜根系硝态氮(NO3--N)和铵态氮(NH4+-N)含量、根系活力、硝酸还原酶(NR)活性、谷氨酰胺合成酶(GS)活性、谷氨酸脱氢酶(GDH)、谷草转氨酶(GOT)活性、谷丙转氨酶(GPT)活性、游离氨基酸和可溶性蛋白质含量总体上均呈现出先升高后降低的变化特点,其中在处理的前20 d液培处理的各项指标均大于雾培,且在处理的第20天达到最大,而雾培处理的各项指标最大值是在处理的第30天,且在处理的30 d之后,雾培根系各项指标均比较高。在不同基因型品种之间,津绿‘21-10’根系各项指标变化幅度大于迷你黄瓜‘22-405’,但品种之间无显著差异。结果说明在生育前期液培黄瓜植株根系氮的吸收和代谢能力比较强,而在生长中后期雾培黄瓜根系氮的吸收和代谢能力增强。

关 键 词:黄瓜  雾培  液培  氮代谢  酶活性

Effect of Aeroponical Culture and Solution Culture on the Root Nitrogen Metabolism Relevant Enzymes of Cucumber
LIU Guangjing,SUN Zhouping,BAO Meili,ZHAO Le and XUE Dongdong.Effect of Aeroponical Culture and Solution Culture on the Root Nitrogen Metabolism Relevant Enzymes of Cucumber[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(3):132-137.
Authors:LIU Guangjing  SUN Zhouping  BAO Meili  ZHAO Le and XUE Dongdong
Institution:LIU Guangjing,SUN Zhouping,BAO Meili,ZHAO Le and XUE Dongdong (Key Laboratory of Protected Horticulture,Ministry of Education,College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang 110866,China)
Abstract:Effects of two different soilless culture systems of aeroponical culture and solution culture(Deep fluid flow) on the root nitrogen metabolism of cucumber plant were studied with two cucumber varieties(Jin green ’21-10’ and mini-cucumber ’22-405’).The results showed that in the whole growth period,there was a tendency and characteristics increased at first and then decreased in the contents of NO3——N,NH4+-N,the free amino acids,soluble protein,and the activity of root vigor,nitrate reductase(NR),glutamine synthase(GS),glutamate dehydrogenase(GDH),glutamate oxaloacetate transaminase(GOT) and glutamate-pyruvate transaminase(GPT) of cucumber root for the aeroponical culture and solution culture,of which in the first of 20 days,all indexes measured in the solution culture were higher than in the aeroponical culture,and reached the maximum at the 20th day;however after treated 30 d,compared with the solution culture,these indexes were higher in aeroponical culture,at the same time reached the maximum at the 30th-40th day.On other hand,between two genotypes,all measured root indexes of Jin green ’21-10’ were higher than those of mini-cucumber ’22-405,but no significant differences.Results showed that during the earlier growing stage,the absorption and metabolism abilities of nitrogen of cucumber root in the solution culture were higher than in aeroponical culture,however,after the middle growth period,these indexes were enhanced in the aeroponical culture.
Keywords:Cucumber  Aeroponical culture  Solution culture  Nitrogen metabolism  
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