| 美洲狮和亚洲黑熊蛔虫ITS序列扩增及序列分析 |
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| 引用本文： | 贺现辉,陈武,何乐天,王培园,袁子国,林瑞庆.美洲狮和亚洲黑熊蛔虫ITS序列扩增及序列分析[J].中国畜牧兽医,2010,37(8):41-44. |
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| 作者姓名： | 贺现辉 陈武 何乐天 王培园 袁子国 林瑞庆 |
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| 作者单位： | （1.华南农业大学兽医学院， 广州 510642； 2.广州动物园， 广州 510070； 3.广州创来生物技术有限公司， 广州 510640） |
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| 基金项目： | 教育部"长江学者和创新团队发展计划"创新团队项目 |
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| 摘 要： | 以采自广州动物园美洲狮和亚洲黑熊体内的蛔虫样品为研究对象,提取样品的总DNA,以保守引物对核糖体DNA内转录间隔区(ITS)序列进行PCR扩增、测序及网上比对。结果表明,美洲狮体内样品获得与GenBankTM公布的狮弓首蛔虫相似性很高的ITS-1、ITS-2和5.8S序列,亚洲黑熊体内样品获得与GenBankTM公布的转移拜林蛔虫相似性很高的ITS-2序列,并首次获得样品的ITS-1与5.8S序列。序列分析结果表明,美洲狮体内蛔虫样品为狮弓首蛔虫,亚洲黑熊体内蛔虫样品为转移拜林蛔虫。
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| 关 键 词： | 美洲狮 亚洲黑熊 蛔虫 核糖体DNA内转录间隔区 序列分析 |
PCR Amplification and Sequence Analysis of Internal Transcribed Spacers (ITS) of Ascarids from Puma concolor and Ursus thibetanus |
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| HE Xian-hui,CHEN WU,HE Le-tian,WANG Pei-yuan,YUAN Zi-guo,LIN Rui-qing.PCR Amplification and Sequence Analysis of Internal Transcribed Spacers (ITS) of Ascarids from Puma concolor and Ursus thibetanus[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):41-44. |
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| Authors: | HE Xian-hui CHEN WU HE Le-tian WANG Pei-yuan YUAN Zi-guo LIN Rui-qing |
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| Institution: | (1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.Guangzhou Zoo, Guangzhou 510070, China; 3.Guangzhou Chuanglai Biotechnology Limited Company, Guangzhou 510640, China) |
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| Abstract: | The DNA internal transcribed spacer (ITS) and 5.8S rDNA of ascaris from the Puma concolor and the Ursus thibetanus in Guangzhou zoo were amplified by PCR respectively using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector. The positive clones identified by PCR amplification were sequenced and analyzed, and compared with relevant sequence in GenBankTM. The results revealed that the ITS and 5.8S sequence of the ascaris from Puma concolor and the ITS-2 sequence from Ursus thibetanus were significantly similar with that of Toxascaris leonina and Baylisascaris transfuga available in GenBankTM respectively. We concluded that the ascaris from Puma concolor represent the T. leonina, and that from Ursus thibetanus represent the B. Transfuga. |
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| Keywords: | Puma concolor Ursus thibetanus ascaris ribosomal DNA internal transcribed spacers sequence analysis |
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