|
|||||
|
|
| 鸡传染性喉气管炎病毒烟台株gB基因的序列测定及其结构功能分析 | |
| 引用本文： | 姬向波,靳双星,陈溥言,管艳萍.鸡传染性喉气管炎病毒烟台株gB基因的序列测定及其结构功能分析[J].中国家禽,2007,29(12). |
| 作者姓名： | 姬向波 靳双星 陈溥言 管艳萍 |
| 作者单位： | 1. 郑州牧业高等专科学校,河南,郑州,450011 2. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 3. 濮阳市兽医卫生监督检验所,河南濮阳,457001 |
| 摘 要： | 研究以鸡传染性喉气管炎病毒(ILTV)烟台株为模板,PCR扩增出1条约2.7kb的gB基因片段,并将其克隆到pMD18-T载体中。序列测定显示,gB基因长2622bp,包含完整的阅读框架。序列比较分析发现,烟台株和王岗株、河北株的gB基因核苷酸序列与SA2株的相比均在第89位上缺失1个碱基G,而在第102位核苷酸处插入1个碱基A,从而引起氨基酸序列中第29～32位5个氨基酸的移码突变。烟台株还有另外7个碱基发生突变,使得其gB基因与河北株、王岗株、疫苗株的gB基因核苷酸同源性分别为99.8%、SA299.7%和99.6%,氨基酸的同源性分别为99.4%、99.4%、98.9%,表明不同ILTV毒株之间gB基因是非常保守的。 |
| 关 键 词： | 鸡传染性喉气管炎病毒 烟台株 糖蛋白gB基因 序列分析 |
Cloning,Sequencing and Structural Analysis of the Glycoprotein B Gene of Infectious Laryngotractheitis Virus Yantai Strain |
|
| Ji Xiangbo,Jin Shangxing,Chen Puyan,Guan Yanping.Cloning,Sequencing and Structural Analysis of the Glycoprotein B Gene of Infectious Laryngotractheitis Virus Yantai Strain[J].China Poultry,2007,29(12). | |
| Authors: | Ji Xiangbo Jin Shangxing Chen Puyan Guan Yanping |
| Abstract: | The glycoprotein B(gB) gene of Yantai strain of infectious laryngotractheitis virus(ILTV) was obtained by polymerase chain reaction and was cloned into pMD18-T vector. The gB gene of Yantai strain was 2622 bp,which contained a complete open reading frame. The results of sequence analysis showed that the homology of the gB gene of Yantai strain with that of Hebei strain,Wanggang strain and SA2 strain were 99.8%,99.7%,99.6%,and the homology of deduced amino acid sequence were 99.4%,99.4%,98.9%,respectively. These data suggested that gB gene was very conserved among different ILTV strains. |
| Keywords: | infectious laryngotractheitis virus Yantai strain glycoprotein B gene sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |