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| 不同高粱自交系幼胚组织培养及植株再生 | |
| 引用本文： | 张美善,赵娜,阎鸿雁,林秀云.不同高粱自交系幼胚组织培养及植株再生[J].吉林农业大学学报,2007,29(3):256-258. |
| 作者姓名： | 张美善 赵娜 阎鸿雁 林秀云 |
| 作者单位： | 1. 吉林农业大学农学院,长春,130118;东北师范大学遗传与细胞研究所分子表观遗传学实验室,长春,130024 2. 东北师范大学遗传与细胞研究所分子表观遗传学实验室,长春,130024 3. 吉林省农业科学院作物研究所,公主岭,136100 4. 东北师范大学遗传与细胞研究所分子表观遗传学实验室,长春,130024;吉林省农业科学院作物研究所,公主岭,136100 |
| 摘 要： | 以7份高粱自交系幼胚为外植体,研究了不同基因型对组织培养的反应和植株再生的差异。结果表明:不同高粱基因型间愈伤组织诱导率没有明显差异,但胚性愈伤组织百分率、不定芽分化率以及再生频率有明显差异。其中YN336A、YN267R、YN510R和YN338A的再生效果较好,其不定芽发生率、不定根发生率及移栽成活率分别为64.4%-72.8%,76.2%-100.0%和36.3%-80.0%。对只生根不生芽的基因型,加入1-2 mg/L KT有明显抑制生根、促进生芽的效应。 |
| 关 键 词： | 高梁 自交系 幼胚 组织培养 植株再生 |
| 文章编号： | 1000-5684（2007）03-0256-03 |
| 修稿时间： | 2006-05-302006-09-10 |
Studies on Tissue Culture and Plant Regeneration of Immature Embryos of Sorghum Inbred Lines |
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| ZHANG Mei-shan,ZHAO Ne,YAN Hong-yan,LIN Xiu-yun.Studies on Tissue Culture and Plant Regeneration of Immature Embryos of Sorghum Inbred Lines[J].Journal of Jilin Agricultural University,2007,29(3):256-258. | |
| Authors: | ZHANG Mei-shan ZHAO Ne YAN Hong-yan LIN Xiu-yun |
| Institution: | 1. College of Agronomy, Jilin Agricultural University, Changchun 130118, China ; 2. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China ; 3. Institute of Crops, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China |
| Abstract: | Explants of immature embryos of seven genotypes of sorghum inbred lines were examined for their response in tissue culture and plant regeneration. The results indicated that there were no significant differences of induction frequency among different genotypes, but the frequency of regenerable callus formation, shoot regeneration and plant regeneration was significantly different. YN336A, YN267R, YN510R and YN338A showed good performances under regeneration culture conditions, frequency of shoot formation, root formation and transplant survive was in the range of 64.4% -72.8 %, 76.2% - 100.0% and 36.3 % - 80.0 %, respectively. For the genotypes that rooted rather than shot, 1 - 2 mg/L KT could effectively inhibit rooting and promote shooting. |
| Keywords: | sorghum inbred line immature embryo tissue culture plant regeneration |
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