| 铁皮石斛组织培养及快繁技术研究 |
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| 引用本文： | 朱庆竖,陈勇,余花,朱碧岩.铁皮石斛组织培养及快繁技术研究[J].中国农学通报,2015,31(31):19-24. |
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| 作者姓名： | 朱庆竖 陈勇 余花 朱碧岩 |
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| 作者单位： | 广东科贸职业学院环境艺术系,华南师范大学生命科学学院,华南师范大学生命科学学院,华南师范大学生命科学学院 |
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| 基金项目： | 广东省高职教育专项资金项目“园艺技术重点专业建设”（粤财教[2014]440 号） |
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| 摘 要： | 为建立铁皮石斛快繁技术体系，给生产提供参考，研究了铁皮石斛组织培养过程中的灭菌方法、腋芽诱导、原球茎的诱导、增殖与分化的最佳培养基配方。以铁皮石斛成熟茎段为外植体，探讨1/2MS培养基或MS培养基中添加不同浓度的激素对铁皮石斛组织培养过程中各生长、分化阶段的影响。研究表明，腋芽诱导最适宜培养基的配方为MS+ 6-BA 2.0 mg/L+ NAA 0.5 mg/L，诱导率达91.28%，平均芽数达2.34；原球茎诱导的最适宜培养基为1/2MS+ 6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L，诱导率可达66.67%；原球茎增殖最佳培养基为1/2MS+ 6-BA 3.0 mg/L+NAA 0.3 mg/L，原球茎分化最适宜培养基为1/2MS+ NAA 1.0 mg/L+ 6-BA 3.0 mg/L+ KT 1.0 mg/L，最适宜生根的培养基配方为1/2MS+NAA 2.0 mg/L+ AC 0.5 g/L。
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| 关 键 词： | 苹果 苹果 水分利用效率 三维树冠 模拟 光合作用 蒸腾作用 |
| 收稿时间： | 2015/7/20 0:00:00 |
| 修稿时间： | 2015/8/24 0:00:00 |
The Study of Culture and Rapid Propagation Technology Dendrobium tissue |
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| Abstract: | In order to establish Dendrobium candidum micropropagation system, for the production of provide a reference of the Dendrobium candidum tissue culture in the process of sterilization methods, axillary bud induction, protocorm like body induction, proliferation and differentiation of the best culture medium formula. In Dendrobium mature stem segments as explants explore, 1 / 2MS medium or MS medium supplemented with different concentrations of hormone axillary bud induction of Dendrobium protocorm induce, proliferation, differentiation and rooting of seedlings, initially built Dendrobium tissue culture propagation technology system. Studies have shown that the most suitable medium axillary bud induction recipe is MS 6-BA 2.0 mg / L NAA 0.5 mg / L, The induction rate reached 91.28%, the average bud number was 2.34；the original bulb induce the most appropriate medium for 1 / 2MS 6-BA 0.5 mg / L NAA 0.5 mg / L 2,4-D 1.0 mg / L, Induction rate of 66.67%;PLB Multiplication most suitable medium for 1 / 2MS 6-BA 3.0mg / L NAA0.3mg / L, PLB optimum differentiation medium for 1 / 2MS NAA1 .0 mg / L 6-BA3.0 mg / L KT1.0mg / L, the most suitable rooting medium was 1 / 2MS 6-BA0mg / L NAA2.0mg / L AC0.5g / L . |
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| Keywords: | Dendrobium officinale axillary bud protocorm hormone |
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