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| 猪精液细管法冷冻保存技术的研究 | |
| 引用本文： | 徐振军,任康,白桂芬,路永强,朱士恩.猪精液细管法冷冻保存技术的研究[J].中国畜牧杂志,2007,43(5):9-11. |
| 作者姓名： | 徐振军 任康 白桂芬 路永强 朱士恩 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京,100094;赤峰学院生命科学系,内蒙古赤峰,024000 2. 中国农业大学动物科技学院,北京,100094 3. 赤峰学院生命科学系,内蒙古赤峰,024000 4. 北京市畜牧兽医总站,北京,100107 |
| 基金项目： | 北京市农委试验示范项目 |
| 摘 要： | 为研制更为简易、高效的冷冻稀释液配方及冷冻程序,充分发挥优良种公猪的生产潜力,本实验采用细管法对种公猪精液冷冻保存技术进行了研究,比较了4种基础液配方稀释液冷冻效果,基础液I:葡萄糖、蔗糖、柠檬酸钠;基础液II:葡萄糖、蔗糖;基础液III:葡萄糖、乳糖、柠檬酸钠;基础液IV:葡萄糖、乳糖。结果表明:采用一步法稀释和IV液冷冻保存猪精液,其解冻后精子活率(0.501)极显著(P<0.01)高于I液(0.359)和III液(0.359),显著(P<0.05)高于II液(0.476),II液显著(P<0.05)高于I液和III液;解冻后IV液的精子顶体完整率(26.9%)显著(P<0.05)高于I液(22.4%)、II液(24.2%)和III液(22.5%),IV液冷冻解冻后精液的精子活率,在室温(23±2)℃下4h内能够保持0.30以上。 |
| 关 键 词： | 精液 细管 超低温冷冻保存 猪 |
| 文章编号： | 0258-7033（2007）05-0009-03 |
| 修稿时间： | 2006-06-142006-07-11 |
Study on Cryopreservation Technique of Boar Semen in Straw |
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| XU Zhen-jun,REN Kang,BAI Gui-fen,LU Yong-qiang,ZHU Shi-en.Study on Cryopreservation Technique of Boar Semen in Straw[J].Chinese Journal of Animal Science,2007,43(5):9-11. | |
| Authors: | XU Zhen-jun REN Kang BAI Gui-fen LU Yong-qiang ZHU Shi-en |
| Institution: | 1. College of Animal Science and Technology, China Agricultural University, Beijing 100094, China; 2. Biology Department of Chifeng University, Inner Mongolia Chifeng 024000, China; 3. General Station of Animal Husbandry and Veterinary Medicine of Beijing, Beijing 100107, China |
| Abstract: | To find the formula of diluents and the technological process, which were simple, convenient, practical and cheaper cost for boar frozen semen, the cryopreservation technique on preserving boar semen in straw was investigated. The cryopreservation effects of four extenders were compared, which the basic chemical compositions were glucose-sucrose-citrate sodium(I), glucose-sucrose(II), glucose-lactose-citrate sodium (III), glucose-lactose (IV). The results showed that among the four kind of diluents, by one step-method diluting, diluent IV is the best one, the motility rate of frozen semen after thawing(0.501) is extremely higher than that in diluent I(0.359)and III(0.359)(P < 0.01), notably higher than diluent II(0.476)(P < 0.05); diluent II is notably higher than that in diluent I and III(P < 0.05); the acrosome-intact rate of frozen semen after thawing(26.9%)is notably higher than that in diluent I(22.4%), II(24.2%)and III(22.5%)(P < 0.05). The motility rate of frozen semen after thawing in diluent IV is over 0.3 within 4 hours at room temperature(23±2)℃. |
| Keywords: | semen straw cryopreservation boar |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |