| 山茱萸中乌索酸和齐墩果酸含量的测定研究 |
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| 引用本文： | 肖道安,邹盛勤.山茱萸中乌索酸和齐墩果酸含量的测定研究[J].安徽农业科学,2007,35(29):9151-9151,9193. |
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| 作者姓名： | 肖道安 邹盛勤 |
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| 作者单位： | 宜春学院化学与生物工程学院,江西宜春,336000;江西省天然药物活性成分研究重点试验室,宜春学院生物工程研究所,江西宜春,336000 |
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| 基金项目： | 国家“863”计划重点资助项目（2002AA223217）. |
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| 摘 要： | 建立反相高效液相色谱法测定了山茱萸中乌索酸和齐墩果酸的含量。色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇∶水∶磷酸(90:10:0.2),流速0.8ml/min;柱温28℃;检测波长为210nm。乌索酸的线性范围0.67～6.03μg,r=0.9996;齐墩果酸的线性范围0.66～5.94μg,r=0.9996。乌索酸和齐墩果酸平均回收率分别为97.6%和99.8%,RSD分别为1.6%和2.1%。方法简便可行,灵敏、准确,重现性好,可作为控制山茱萸药材质量的检测方法。
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| 关 键 词： | 山茱萸 乌索酸 齐墩果酸 反相高效液相色谱法 含量测定 |
| 文章编号： | 0517-6611（2007）29-09151-01 |
| 修稿时间： | 2007-05-11 |
Study on Content Determination of Ursolic Acid and Oleanolic Acid in Comus officinalisis |
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| XIAO Dao-an et al.Study on Content Determination of Ursolic Acid and Oleanolic Acid in Comus officinalisis[J].Journal of Anhui Agricultural Sciences,2007,35(29):9151-9151,9193. |
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| Authors: | XIAO Dao-an |
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| Institution: | College of Chemistry and Bioengineering, Yichun University, Yichun, Jiangxi 336000 |
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| Abstract: | A RP-HPLC method for content determination of ursolic acid and oleanolic acid in Cornus officinalis was established.The Kromasil C18(250 mm×4.6 mm,5 μm) was used as analytical column with methanol-water-phosphate acid(90∶10∶0.2) as mobile phase.The detection wavelength was set at 210nm.The flow rate was 0.8 ml/min and the column temperature was 28 ℃.The calibration curve of ursolic acid was linear over the range of 0.67～6.03 μg and the correlation coefficient was 0.999 6.The calibration curve of oleanolic acid was linear over the range of 0.66～5.94 μg and the correlation coefficient was 0.999 6.The average recoveries of ursolic acid and oleanolic acid were 97.6 %(RSD= 1.6 %) and 99.8 %(RSD=2.1%),respectively.The method was simple,sensitive,accurate and reproducible.It could be used to control the quality of Cornus officinalis. |
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| Keywords: | Cornus officinalis Ursolic acid Oleanolic acid RP-HPLC Content determination |
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