| 黄瓜枯萎病病原拮抗细菌DS-1菌株鉴定及其生防效果研究 |
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| 引用本文： | 张璐,丁延芹,杜秉海,魏珉,王秀峰.黄瓜枯萎病病原拮抗细菌DS-1菌株鉴定及其生防效果研究[J].园艺学报,2010,37(4):575-580. |
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| 作者姓名： | 张璐 丁延芹 杜秉海 魏珉 王秀峰 |
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| 作者单位： | （1山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，山东泰安271018；2山东农业大学生命科学学院，山东泰安271018） |
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| 基金项目： | 国家科技支撑计划重点项目，山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 采用平板对峙法，从日光温室连作黄瓜根际土壤中筛选出对黄瓜枯萎病病原菌（Fusarium oxysporum f. sp. cucumerinum）具有稳定拮抗作用的细菌菌株DS-1，通过形态观察、生理生化测定和16S rDNA序列分析对该菌株进行了鉴定，并研究了对黄瓜种子萌发的影响和对苗期枯萎病的生防效果。结果表明：菌株DS-1与已报道的Brevibacillus brevis 16S rDNA具有100%同源性，结合形态特征和生理生化特性，确定DS-1为短短芽孢杆菌。基因库中该菌株的序列登录号为EF108303。DS-1对黄瓜种子萌发具有促生作用，苗期防治枯萎病的效果显著，防效达84.92%。
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| 关 键 词： | 黄瓜 黄瓜枯萎病病原菌 拮抗细菌 鉴定 Brevibacillus brevis |
| 收稿时间： | 2009-11-24 |
Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum |
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| ZHANG Lu,DING Yan-qin,DU Bing-hai,WEI Min,WANG Xiu-feng.Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum[J].Acta Horticulturae Sinica,2010,37(4):575-580. |
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| Authors: | ZHANG Lu DING Yan-qin DU Bing-hai WEI Min WANG Xiu-feng |
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| Institution: | （1College of Horticultural Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology， Tai’an，Shandong 271018，China；2College of Life Sciences，Shandong Agricultural University，Tai’an，Shandong 271018，China） |
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| Abstract: | The taxonomic status, growth promoting and biocontrol effects against Fusarium oxysporum f. sp. cucumerinum of antagonistic bacterium DS-1 strain, screened by co-culture on PDA plates from rhizospheric soil of continuously cropped cucumber in solar greenhouse, morphology, physiological and biochemical characteristics, and 16S rDNA sequencing of the strain were determined, the effects on cucumber seed germination and seedling fusarium wilt biocontrol were studied. The results showed that: The 16S rDNA sequen... |
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| Keywords: | Brevibacillus brevis cucumber Fusarium oxysporum f sp cucumerinum antagonistic bacterium identification Brevibacillus brevis |
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