| 荷斯坦奶牛耐热性状的分子遗传标记研究 |
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| 引用本文： | 龚泽修,肖定福.荷斯坦奶牛耐热性状的分子遗传标记研究[J].浙江畜牧兽医,2007,32(3):6-8. |
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| 作者姓名： | 龚泽修 肖定福 |
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| 作者单位： | 1. 湖南省娄底市生物机电工程学校,湖南娄底,417000
2. 湖南农业大学动物科学与技术学院 |
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| 摘 要： | 选取高产荷斯坦牛10头，优化奶牛RAPD反应最佳体系，并在154条随机引物中筛选出稳定性好、带型清晰的引物64条。结合红细胞钾测定结果和生产性能测定，通过RAPD分析并筛选出3个与奶牛耐热性状相关的分子标记，以期应用这些分子标记进行高产耐热荷斯坦奶牛的选育工作。
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| 关 键 词： | 耐热性状 分子标记 扩增多态性 荷斯坦奶牛 |
| 文章编号： | 1005-7307（2007）03-0006-003 |
| 收稿时间： | 2007-03-16 |
| 修稿时间： | 2007年3月16日 |
Study of Molecular Genetic Markers for Heat Resistant Traits in Holstein Dairy Cattle |
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| Gong Zexiu,Xiao Dingfu.Study of Molecular Genetic Markers for Heat Resistant Traits in Holstein Dairy Cattle[J].Zhejiang Journal of Animal Science and Veterinary Medicine,2007,32(3):6-8. |
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| Authors: | Gong Zexiu Xiao Dingfu |
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| Institution: | 1. Loudi biology and electromechanical engineer school scince, HuNan,LouDi ,417000; 2. College of animal science and technology, Hunan Agricultural University, HuNan, Changsha ,410128 |
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| Abstract: | In this paper, with 10 high yield Holstein Cattle as materials, we was optimized RAPD reaction system, and 64 primers with better result were selected in 154 random primers. Considered the detected result of potassium content in erythrocyte and production performance, three molecular markers relative to heat-tolerance in Holstein Cattle were selected. We expect the research that helps to establish high-yield and high heat tolerance dairy cattle breed. |
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| Keywords: | Holstein Cattle RAPD Heat-tolerance molecular marker |
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