| 甘蓝型油菜白花基因的RAPD标记 |
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| 引用本文： | 董育红,田建华,李殿荣.甘蓝型油菜白花基因的RAPD标记[J].西北农林科技大学学报(社会科学版),2005,33(10):57-61. |
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| 作者姓名： | 董育红 田建华 李殿荣 |
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| 作者单位： | [1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]陕西省杂交油菜研究中心,陕西大荔715105 |
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| 基金项目： | 陕西省自然科学研究计划项目E2001SM32] |
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| 摘 要： | 以甘蓝型白花油菜及其分离群体为材料,对甘蓝型油菜白花性状的遗传规律进行了研究。结果表明,白花对黄花是一对由不完全显性基因(WW)控制的不完全显性遗传性状;利用集群分离法(BSA)对该白花基因进行RAPD分析,在1 200个随机引物中,获得了引物S1092(5-CCC AGG CTA C-3),在白花基因库和黄花基因库间扩增出多态性产物S-10921250;对该分离群体及其姊妹系和其他白花油菜进行的单株验证表明,S-10921250与甘蓝型油菜白花基因相连锁,遗传距离为0.84 cM。
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| 关 键 词： | 甘蓝型油菜 白花 分子标记 RAPD 集群分离法 |
| 文章编号： | 1671-9387（2005）10-0057-05 |
| 收稿时间： | 2005-06-23 |
| 修稿时间： | 2005年6月23日 |
RAPD markers linked to white-petal gene in Brassica napus L. |
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| DONG Yu-hong,TIAN Jian-hua,LI Dian-rong, GUO Ai-guang,KONG Jian,ZHAO Xiao-ping,.RAPD markers linked to white-petal gene in Brassica napus L.[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(10):57-61. |
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| Authors: | DONG Yu-hong TIAN Jian-hua LI Dian-rong GUO Ai-guang KONG Jian ZHAO Xiao-ping |
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| Institution: | DONG Yu-hong,TIAN Jian-hua,LI Dian-rong, GUO Ai-guang,KONG Jian,ZHAO Xiao-ping, ( 1 College of Life Sciences, Northwest A |
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| Abstract: | White-petal(W) rapeseed(Brassica napus L.) and its segregation population were used as experiment materials to study the inheritance of its white-petal traits.The result showed that the petal color character of W vs Y(yellow-petal) was controlled by one pair incomplete dominant gene WW.Bulked segregation analysis(BSA) was employed to identify random amplified polymorphic DNA(RAPD) markers linked to the white-petal gene.A total of 1 200 arbitrary 10-mer oligonucleotide primers were screened on the DNA of W and Y bulks.Primer S_(1092)(5CCC AGG CTA C-3) gave polymorphism between the two bulks.DNA from Individual plants of the two bulks,their sister lines and the other white-petal rapeseed(Brassica napus L.) were then used as template for screening with the primer S_(1092) and the 1.25 kb DNA polymorphism generated by the primer S_(1092) were verified linking with the white-petal gene.The genetic distance was 0.84 cM between RAPD marker and white-petal gene. |
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| Keywords: | Brassica napus L ~ white-petal ~ molecular marker ~ RAPD ~ BSA |
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