| 用组织培养法进行马铃薯体细胞染色体的加倍 |
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| 引用本文： | 王清.用组织培养法进行马铃薯体细胞染色体的加倍[J].甘肃农业大学学报,1996,31(2):155-159. |
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| 作者姓名： | 王清 |
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| 作者单位： | 甘肃农业大学农学系 |
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| 摘 要： | 用马铃薯普通栽培种的２个一单倍体、２个纯合二倍体和５个双单倍体材料，以半叶、茎、叶柄作为外植体通过先诱导愈伤组织，再分化苗的两步法进行了染色体的加倍实验。结果表明：用组织培养二步法可以获得较高的加倍率；加倍率高达９３．３３％。成愈率、苗分化率和加倍率均以半叶为最高，其次为茎段和叶柄；苗分化率的高低直接影响加倍率，二者呈显著的正相关（ｒ＝０．７３３６）；适当地延长愈伤组织培养时间有利于提高加倍率，本实验结果以４０ｄ为宜；基因型对成愈率、苗分化率及加倍率均有影响，亲缘关系近的材料这三种频率也相近；倍性低的材料加倍率较高。
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| 关 键 词： | 马铃薯一单倍体 双单倍体 染色体加倍 |
Studies on The Techniques of Doubling Somatic Cell Chromosome in Solanum Tuberosum L.BY Tissue Culture |
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| Wang Qing.Studies on The Techniques of Doubling Somatic Cell Chromosome in Solanum Tuberosum L.BY Tissue Culture[J].Journal of Gansu Agricultural University,1996,31(2):155-159. |
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| Authors: | Wang Qing |
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| Abstract: | The stem and petiole segments and half-leaf pieces from 5 dihaploids,2 monohaploids and 2 doubled monohaploids were used as the explants in vitro culture to induce calli and shoots.The results indicated that the two-step tissue culture method could obtain high frequency of chromosome doubling,the highest one was 93 33%.The freqencies of callus induction,shoot regeneration and chromosome doubling from half-leaf were higher than that from stem and petiole segments.The chromosome doubling percentage of the shoots was directly affected by the freqency of shoot regeneration,there was a significant possitive correlation between them (r=0 7336).Lengthening callus culture time could increase the frequency of doubling shoots,the frequencies of callus induction,shoot regeneration and chromosome doubling.However,the 3 frequencies had no significant difference among the close relative materials .The lower ploidy materials,the higher chromosome doubling frequency. |
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| Keywords: | Solanum tuberosum L somatic cell chromosomedoubling tissue calture |
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