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| MSAP技术及其在橡胶树遗传育种研究中的应用前景 | |
| 引用本文： | 吴春太,李维国,高新生,张晓飞.MSAP技术及其在橡胶树遗传育种研究中的应用前景[J].贵州农业科学,2009,37(11). |
| 作者姓名： | 吴春太 李维国 高新生 张晓飞 |
| 作者单位： | 1. 中国热带农业科学院,橡胶研究所,海南,儋州,571737 2. 国家橡胶树育种中心,海南,儋州,571737 3. 农业部热带作物栽培生理学重点实验室,海南,儋州,571737 |
| 基金项目： | 海南省自然科学基金项目"橡胶芽接树接穗DNA的甲基化分析" |
| 摘 要： | MSAP是一种基于限制性内切酶的新技术,结合了扩增片段长度多态性的优点,已被广泛应用于植物种质资源鉴定、品种改良、种质资源表观遗传多样性分析、基因表达调控、杂种优势预测与利用、转基因沉默、种群表观遗传结构分析及环境适应研究等遗传育种各个领域.对MSAP技术的原理、基本程序、方法的评价以及其在橡胶树遗传育种研究中应用的重要性进行了综述,并对其应用前景进行了展望. |
| 关 键 词： | DNA甲基化 DNA甲基化敏感扩增多态性(MSAP) 橡胶树 遗传育种 |
Methylation-sensitive Amplified Polymorphism and Its Application Prospect in Hereditary and Breeding of Rubber Trees |
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| WU Chun-tai,LI Wei-guo,GAO Xin-sheng,ZHANG Xiao-fei.Methylation-sensitive Amplified Polymorphism and Its Application Prospect in Hereditary and Breeding of Rubber Trees[J].Guizhou Agricultural Sciences,2009,37(11). | |
| Authors: | WU Chun-tai LI Wei-guo GAO Xin-sheng ZHANG Xiao-fei |
| Institution: | WU Chun-tai,LI Wei-guo,GAO Xin-sheng,ZHANG Xiao-fei(1.Rubber Research Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou,Hainan 571737,2.State Center for Rubber Breeding,3.Key Laboratory for Tropical Crops Physiology,Ministry of Agriculture,China) |
| Abstract: | MSAP,a new technology based on restriction enzyme with the advantage of amplified section length polymorphism,has being widely applied in identification of plant germplasm resources,variety improvement,apparent genetic diversify analysis of germplasm resources,gene expression adjustment,forecast and utilization of heterosis,trans-gene silent,population apparent genetic construction analysis and environment adaptability.The paper reviews the principle,basic process and evaluation of MSAP,and the importance i... |
| Keywords: | DNA methylation methylation-sensitive amplified polymorphism (MSAP) rubber tree genetic and breeding |
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