| 日本鳗鲡及其僵鳗消化酶活性的比较研究 |
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| 引用本文： | 黄永春.日本鳗鲡及其僵鳗消化酶活性的比较研究[J].动物营养学报,2012(5):977-982. |
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| 作者姓名： | 黄永春 |
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| 作者单位： | 集美大学水产学院，厦门 ３６１０２１ |
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| 基金项目： | 李尚大集美大学学科建设基金（ＺＣ２０１０００１） |
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| 摘 要： | 本试验旨在比较不同反应温度下日本鳗鲡(Anguilla japonics)及其僵鳗消化酶活性的差异.试验选取正常生长的日本鳗鲡(126.4～140.2g/尾)以及生长缓慢的僵鳗(3.5～8.6 g/尾)各20尾,取肝胰脏、胃、肠,分别在5、15、25、30、35、40、45和55℃反应温度下测定肝胰脏、胃、肠中蛋白酶、...
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| 关 键 词： | 温度 日本鳗鲡 僵鳗 消化酶活性 |
Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels(Anguilla japonics) |
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| HUANG Yongchun.Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels(Anguilla japonics)[J].Acta Zoonutrimenta Sinica,2012(5):977-982. |
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| Authors: | HUANG Yongchun |
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| Institution: | HUANG Yongchun(Fishery College,Jimei University,Xiamen 361021,China) |
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| Abstract: | This experiment was conducted to study the effects of temperatures on the activities of digestive enzymes in Japanese eels Anguilla japonics(n=20) and growth retarded eels(n=20).The weight of the normal eels was from 126.4 to 140.2 g per eel,and that of growth retarded eels was from 3.5 to 8.6 g per eel.The activities of protease,amylase,and lipase in the normal eels and growth retarded eels under the condition of different temperatures(5,10,15,25,30,35,40,45 and 55 ℃) were studied.The results showed as follows: with the increasing of temperature,the digestive enzyme activities were increased at first,and then decreased.The optimum temperature of the protease in hepatopancreas,intestine and stomach was 45 ℃.In the same experimental temperature,the order of protease activity was intestine>stomach>hepatopancreas except growth retarded eels at 5,15 and 55 ℃.The protease activity in hepatopancreas,intestine and stomach of growth retarded eels was only 29.5%,15.7% and 25.2% of that of the normal eels,respectively(P<0.05).The optimum temperature of amylase activity was 30 ℃,and the order of amylase activity of the normal eels was hepatopancreas>intestine>stomach in experimental temperatures except at 5 ℃,but the order of amylase activity of growth retarded eels was intestine>hepatopancreas>stomach in experimental temperatures.The amylase activity in hepatopancreas,intestine and stomach of growth retarded eels was only 42.4%,73.7% and 43.8% of that of the normal eels,respectively(P<0.05).The optimum temperature of lipase activity was 35 ℃,and the order of lipase activity of the normal eels was hepatopancreas>stomach>intestine in experimental temperatures,but the order of lipase activity of growth retarded eels was hepatopancreas>intestine>stomach in experimental temperatures except at 35 ℃;the lipase activity in hepatopancreas,intestine and stomach of growth retarded eels was only 41.5%,45.6% and 23.2% of that of the normal eels,respectively(P<0.05).These results indicate that the activities of protease,amylase and lipase in hepatopancreas,intestine and stomach of growth retarded eels are significantly lower than those of normal eels,which directly affect their growth and development. |
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| Keywords: | temperature Anguilla japonics growth retarded eels digestive enzyme activities |
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