| 共轭亚油酸对蛋鸡生产性能、蛋壳质量和血清生化指标的影响 |
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| 引用本文： | 刘雪兰,石天虹,井庆川,阎佩佩,魏祥法,刘瑞亭.共轭亚油酸对蛋鸡生产性能、蛋壳质量和血清生化指标的影响[J].动物营养学报,2012(5):926-932. |
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| 作者姓名： | 刘雪兰 石天虹 井庆川 阎佩佩 魏祥法 刘瑞亭 |
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| 作者单位： | （山东省农业科学院家禽研究所，济南 ２５００２３） |
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| 基金项目： | 山东省自然科学基金资助项目（ＺＲ２００９ＤＱ０１７） |
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| 摘 要： | 为了研究共轭亚油酸（ＣＬＡ）对蛋鸡生产性能和蛋壳质量的影响及机理，试验选用 ４０周龄健康、体重和产蛋率相近的海兰白蛋鸡 ４８０只，采用单因素试验设计，随机分为 ４组，每组 ３个重复，每个重复４０只，试验组分别添加０．５％、１．０％和２．０％的 ＣＬＡ，对照组不添加，试验期８周。试验结束时，每组选 ６只采血并屠宰，测定血清中三碘甲腺原氨酸（Ｔ３）、四碘甲腺原氨酸（Ｔ４）、皮质醇、雌二醇（Ｅ２）、钙离子、磷离子含量和碱性磷酸酶（ＡＬＰ）活性及肝脏肉毒碱棕榈酰转移酶（ＣＰＴ Ⅰ）活性。结果表明：１）各试验组蛋鸡生产性能与对照组均差异不显著（Ｐ＞０．０５）。２）各试验组蛋壳强度均显著大于对照组（Ｐ＜０．０５），蛋壳厚度均差异不显著（Ｐ＞０．０５）。３）２．０％ＣＬＡ组蛋鸡血清 Ｔ３、Ｔ４、皮质醇和 Ｅ２含量显著高于对照组及 ０．５％和 １．０％ＣＬＡ组（Ｐ＜０．０５）；各试验组蛋鸡血清钙、磷离子浓度与对照组差异不显著（Ｐ＞０．０５）；０．５％和 １．０％ＣＬＡ组蛋鸡血清 ＡＬＰ活性显著高于对照组（Ｐ＜０．０５）。４）１．０％和 ２．０％ＣＬＡ组蛋鸡肝脏 ＣＰＴ Ⅰ活性显著低于对照组（Ｐ＜０．０５）。由此可见，在本试验条件下，饲粮添加 １．０％的ＣＬＡ能显著提高蛋鸡血清中 ＡＬＰ活性，并在一定程度上促进 Ｅ２的分泌，显著提高蛋壳强度，且不影响蛋鸡的生产性能。
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| 关 键 词： | ＣＬＡ 生产性能 蛋壳质量 血清生化指标 ＡＬＰ 内分泌激素 ＣＰＴ Ⅰ |
Dietary Conjugated Linoleic Acid Level Affects Performance,Eggshell Quality and Serum Biochemical Indices of Laying Hens |
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| LIU Xuelan,SHI Tianhong,JING Qingchuan,YAN Peipei,WEI Xiangfa,LIU Ruiting.Dietary Conjugated Linoleic Acid Level Affects Performance,Eggshell Quality and Serum Biochemical Indices of Laying Hens[J].Acta Zoonutrimenta Sinica,2012(5):926-932. |
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| Authors: | LIU Xuelan SHI Tianhong JING Qingchuan YAN Peipei WEI Xiangfa LIU Ruiting |
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| Institution: | (Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan 250023,China) |
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| Abstract: | This experiment was conducted to investigate the effects of the supplementation of conjugated linoleic acid(CLA) on the performance,eggshell quality,and its mechanism.Four hundred and eighty healthy 40-week-old Hy-Line White laying hens with similar body weight and laying rate were divided randomly into 4 groups with 3 replicates per group and 40 birds in each replicate.The control diet had no CLA,and the experimental diets contained 0.5%,1.0% and 2.0% CLA,respectively.Eight weeks later,six of each group was slaughtered and the blood was collected to determine the contents of thiothyone(T3),tetraiodothyronine(T4),cortisol,estradiol(E2),Ca2+and P5+,the alkaline phosphatase activity(ALP) in serum,and the carnitine palmitoyltransterase(CPT-Ⅰ) activity in liver.The results showed as follows: 1) there was no significant difference in performance between the control group and experimental groups(P>0.05).2) Compared with the control group,the eggshell strength in experimental groups was significantly improved(P<0.05),but there was no significant difference in eggshell thickness between the control group and experimental groups(P>0.05).3) The contents of T3,T4,cortisol and E2 in serum of laying hens in 2.0% CLA group were significant higher than those in the other groups(P<0.05);there were no significant differences in the contents of calcium ions and inorganic phosphorus ions in serum of laying hens between the control group and experimental groups(P>0.05);compared with the control group,the ALP activity in serum of laying hens in experimental groups was significantly increased(P<0.05) except for 2.0% CLA group.4) The CPT-Ⅰ activity in liver of laying hens was significantly lower than that in the control group(P<0.05).It is concluded that 1.0% CLA can significantly increase serum ALP activity,and improve serum E2 content,so it can significantly improve the eggshell strength,furthermore,it has no bad effect on performance of layers. |
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| Keywords: | conjugated linoleic acid performance eggshell quality serum biochemical indices alkaline phosphates endocrine hormones carnation palmitoyltransterase |
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