| 稻瘟病抗性基因Pi25特异性CAPS标记的开发与验证 |
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| 引用本文： | 王惠梅**,陈洁**,施勇烽,潘刚,沈海超,吴建利.稻瘟病抗性基因Pi25特异性CAPS标记的开发与验证[J].作物学报,2012,38(11):1960-1968. |
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| 作者姓名： | 王惠梅** 陈洁** 施勇烽 潘刚 沈海超 吴建利 |
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| 作者单位： | 1.中国水稻研究所 / 水稻生物学国家重点实验室, 浙江杭州 310006;2.浙江大学农业与生物技术学院, 浙江杭州 310058 |
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| 基金项目： | This work was supported by the National High Technology Research and Development Program of China (Grant No. 2011AA10A101 and 2012AA101102) and the Ministry of Finance, China (Grant No. 2012RG002-4). |
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| 摘 要： | 为在水稻育种中快速与高效利用稻瘟病抗性基因Pi25, 本文利用该基因不同等位基因编码区序列差异开发了4套CAPS标记(CAP1/Hinc II、CAP3/Bgl II、CAP3/Nde I和CAP3/Hpy 99I), 并利用169份稻种资源、98个重组自交系(RIL)以及217个水稻转基因后代, 对4套标记的准确性和选择效果进行了验证。结果表明, 4套标记均能准确地检测Pi25/pi25座位。其中, 标记CAP1/Hinc II和CAP3/Hpy 99I特异性识别并酶切显性等位基因, 而标记CAP3/Bgl II和CAP3/Nde I特异性识别并酶切隐性等位基因。利用稻瘟病菌株JS001-20接种RIL与转基因材料, 抗性表现与标记检测的结果完全一致, 表明该CAPS标记准确可靠。分析稻种资源后发现, Pi25基因频率较低(1.2%), 说明该基因在我国水稻稻瘟病抗性育种中还没有被充分利用。本文的研究结果特别是开发的2对识别并酶切显性等位基因的CAPS标记可用于分子标记辅助选择, 改良我国早籼稻的稻瘟病抗性。
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| 关 键 词： | 水稻 稻瘟病抗性 酶切扩增多态性序列 标记辅助选择 单核苷酸多态性 |
| 收稿时间： | 2012-04-17 |
Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene Pi25 |
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| WANG Hui-Mei,CHEN Jie,SHI Yong-Feng,PAN Gang,SHEN Hai-Chao,WU Jian-Li.Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene Pi25[J].Acta Agronomica Sinica,2012,38(11):1960-1968. |
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| Authors: | WANG Hui-Mei CHEN Jie SHI Yong-Feng PAN Gang SHEN Hai-Chao WU Jian-Li |
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| Institution: | 1.State Key Laboratory of Rice Biology / China National Rice Research Institute, Hangzhou 310006, China;2.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | To promote the application of rice blast resistance gene Pi25 in rice breeding programs, we developed four sets of gene-specific CAPS markers (CAP1/Hinc II, CAP3/Bgl II, CAP3/Nde I, and CAP3/Hpy 99I) based on the coding sequences of the locus. One hundred and sixty-nine rice accessions, 98 recombinant inbred lines (RILs) and 217 transgenic plants were used for the validation of the markers. The results showed that all the four sets of markers were able to accurately and efficiently detect the Pi25/pi25 locus, CAP1/Hinc II and CAP3/Hpy 99I could digest specifically the dominant allele Pi25 while CAP3/Bgl I and CAP3/Nde I were able to digest specifically the recessive allele pi25. RILs and transgenic lines carrying Pi25 allele were resistant to the blast isolate JS001-20 while the lines carrying pi25 allele were susceptible, indicating a perfect detection of the target locus by the CAPS markers. In addition, a low frequency (1.2%) of the dominant allele was detected in the germplasm collections, indicating this gene has not been fully utilized in rice breeding programs in China. Markers CAP1/Hinc II and CAP3/Hpy 99I are recommended and will be useful for the improvement of blast resistance, especially for the early-season indica rice. |
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| Keywords: | Oryza sativa Blast resistance Cleaved amplified polymorphic sequence (CAPS) Marker-assisted selection (MAS) Single nucleotide polymorphism (SNP) |
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