| 间歇浸没式生物反应器进行甘蔗脱毒苗快繁研究 |
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| 引用本文： | 刘丽敏,李松,余坤兴,唐红琴,刘红坚,淡明,卢曼曼,戴友铭.间歇浸没式生物反应器进行甘蔗脱毒苗快繁研究[J].农业科学与技术,2010(5). |
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| 作者姓名： | 刘丽敏 李松 余坤兴 唐红琴 刘红坚 淡明 卢曼曼 戴友铭 |
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| 作者单位： | 广西甘蔗研究所,广西南宁,530007 |
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| 基金项目： | 广西科学基金项目，广西农业科学院科技发展资金项目(2006006).Supported by Youth Science Foundation of Guangxi，S&T Development Project from Guangxi Academy of Agricultural Sciences |
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| 摘 要： | 利用新型间歇浸没式生物反应系统(TIBs)对2个甘蔗品种ROC16和ROC22的脱毒苗进行快繁研究,在培养基配方、接种数量、蔗糖浓度及品种差异等方面进行筛选和比较.结果表明,利用该系统,ROC16增殖率达40倍,每瓶生产组培苗800株苗左右;ROC22增殖率为30倍,每瓶苗的数量达400～600株.该研究为间歇浸没式生物反应系统应用于组培快繁提供了依据.
Abstract：
By employing temporary immersion bioreactor system (TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference.The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets;of ROC22 improved by 30 times,per flask generated about 400-600 plantlets.The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.
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| 关 键 词： | 间歇 浸没式 生物反应器 甘蔗品种 脱毒苗快繁 proliferation rate rapid propagation 反应系统 improved results culture 增殖率 培养基配方 flask 组培快繁 蔗糖浓度 系统应用 品种差异 provided variety |
Rapid Propagation of Virus-free Sugarcane Plantlets via Temporary Immersion Bioreactor System |
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| Abstract: | Seccharrum officinarurn L. Temporary immersion bioreactor system Virus-free seedling Rapid propagation |
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| Keywords: | Seccharrum officinarurn L Temporary immersion bioreactor system Virus-free seedling Rapid propagation |
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