| 不同采胶强度下橡胶树胶乳C-乳清蛋白的双向电泳初析 |
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| 引用本文： | 肖再云,刘实忠,校现周.不同采胶强度下橡胶树胶乳C-乳清蛋白的双向电泳初析[J].热带作物学报,2009,30(7):897-901. |
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| 作者姓名： | 肖再云 刘实忠 校现周 |
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| 作者单位： | 1. 海南大学园艺园林学院,海南儋州,571737;中国热带农业科学院橡胶研究所,海南儋州,571737;云南省热带作物科学研究所,云南景洪,666100
2. 海南大学园艺园林学院,海南儋州,571737;中国热带农业科学院橡胶研究所,海南儋州,571737 |
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| 基金项目： | 基本科研业务专项资金 |
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| 摘 要： | 选取采胶强度分别为严重过度、适中、严重不足的橡胶树胶乳,高速离心分离得到C-乳清,提取C-乳清中的蛋白质进行双向凝胶电泳,并通过PDQuest软件分析比较.结果表明:采胶强度严重不足的与其它采胶强度的相比,橡胶树胶乳C-乳清的差异表达蛋白质点有17个,其中,14个点相对上调,3个点相对下调:采胶强度适中的与其它采胶强度的相比,橡胶树胶乳C-乳清的差异表达蛋白点质有12个,其中,5个点相对上调,7个点相对下调;采胶严重过度的与其它采胶强度的相比,橡胶树胶乳C-乳清的差异表达蛋白质点有14个,其中,8个点相对上调,6个点相对下调;随着采胶强度的增加而明显上调的蛋白质点有1个:发现采胶强度高的橡胶树较采胶强度低的橡胶树胶乳C-乳清蛋白质下调的数量更多,上调的数量更少.并且采胶强度相差越大.下调与上调的比例也越大.通过研究不同采胶强度下的橡胶树胶乳C-乳清蛋白质差异,找出采胶强度与胶乳C-乳清蛋白质的关系,并利用这种关系进行乙烯刺激采胶强度的判断,进而指导采胶生产和研究.
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| 关 键 词： | 橡胶树 采胶强度 C-乳清 蛋白质 双向电泳 |
Two-dimensional Gel Electrophoresis of C-serum Proteins from Hevea Latex |
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| Xiao Zaiyun,Liu Shizhong and Xiao Xianzhou.Two-dimensional Gel Electrophoresis of C-serum Proteins from Hevea Latex[J].Chinese Journal of Tropical Crops,2009,30(7):897-901. |
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| Authors: | Xiao Zaiyun Liu Shizhong and Xiao Xianzhou |
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| Institution: | Xiao Zaiyun1,2,3,Liu Shizhong1,Xiao Xianzhou1,21 College of Horticulture , L,scape Architecture,Hainan University,Danzhou,Hainan 571737,2 Rubber Research Institute,Chinese Academy of Tropical Sciences,3 Yunnan Institute of Tropical Crops,Jinghong,Yunnan 666100 |
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| Abstract: | Latex was collected from rubber trees which were over-exploited,moderate-exploited or under-exploited.The C-serum of the latex was isolated by ultra-centrifuge,and subsequently the proteins were extracted and applied to two-dimensional gel electrophoresis.The resultant data were analyzed by using the PDQuest Software.Seventeen differentially expressed protein spots in the 2-D gel of latex protein were found from the under-exploited rubber trees,of which 14 were up-regulated and 3 down-regulated;12 different... |
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| Keywords: | tapping intensity c-serum protein two-dimensional gel electrophoresis rubber tree |
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