| 杜松烯合成酶作为转基因棉花PCR检测的内参照基因 |
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| 引用本文： | 黄文胜,赵文军,陈红运,陈颖,徐宝梁,朱水芳.杜松烯合成酶作为转基因棉花PCR检测的内参照基因[J].植物检疫,2006,20(1):5-7. |
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| 作者姓名： | 黄文胜 赵文军 陈红运 陈颖 徐宝梁 朱水芳 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100025 |
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| 摘 要： | 根据棉属植物中棉酚合成的关键酶之一杜松烯合成酶（＋）-d-cadinene syhnthase]基因序列，设计合成了该酶的实时荧光PCR的引物和TaqMan探针，经研究发现，此套引物探针能特异性检测海岛棉和陆地棉，有较高的检测灵敏度。同马铃薯、大椒、茄子、烟草、番茄、玉米、大豆、小麦、水稻和其它转基因作物无非特异性反应，可以作为转基因棉花检测的内参照。
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| 关 键 词： | 转基因棉花 转基因检测 实时荧光PCR |
| 收稿时间： | 2005-03-03 |
| 修稿时间： | 2005年3月3日 |
Validation of a cotton specific gene, ( + )-d-cadinene synthase, used as the endogenous reference gene for real-time PCR transgenic detection |
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| Huang Wensheng,Zhao Wenjun,Chen Hongyun,Chen Ying,Xu Baoliang,Zhu Shuifang.Validation of a cotton specific gene, ( + )-d-cadinene synthase, used as the endogenous reference gene for real-time PCR transgenic detection[J].Plant Quarantine,2006,20(1):5-7. |
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| Authors: | Huang Wensheng Zhao Wenjun Chen Hongyun Chen Ying Xu Baoliang Zhu Shuifang |
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| Institution: | Chinese Academy of Inspection and Quarantine, Beijing 100025, China |
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| Abstract: | Many countries have issued regulations to label the genetically modified organisms(GMOs)and their derived products.In this article,we reported the development of specific primers and probe for the cotton(Gossypium hirsutum)(+)-d-cadinene synthase gene and PCR cycling conditions suitalbe for which use as an endogenous reference gene in PCR assays.Expect results were obtained when method was assayed with 7 different cotton varieties,and no amplification were observed when other species,such as wheat,maize,tobecco,soybean,rice,tomato,pepper,eggplant were used as templates,which demonstrated the(+)-d-cadinene synthase gene was cotton specific,had low heterogeneity among the tested cultivars.Therefore,this gene could be used as an endogenous reference gene of cotton in practial real time transgenic detection. |
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| Keywords: | Genetically modified cotton transgenic detection real time florescent PCR |
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