Chloroplast DNA variation in cultivated and wild Prunus avium L: a comparative study |
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| Authors: | S Panda J P Martin I Aguinagalde A Mohanty |
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| Institution: | Departamento de Biologia Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, E-28040 Madrid, Spain;International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067 New Delhi, India |
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| Abstract: | Chloroplast DNA variation in 96 Prunus avium L. cultivars was assessed and compared with the results of a previous study of cpDNA diversity in 23 wild populations of the species. The polymerase chain reaction‐restriction fragment length polymorphism method was used in these studies. Approximately 9% of the chloroplast genome was analyzed, using five universal primer pairs and three restriction enzymes. Ten polymorphic fragments were common to both the wild and sweet cherry; eight polymorphic fragments were found only in the wild cherry. In the cultivars, all mutations were small (5‐30 bp) indels. In the wild populations, a point mutation was also detected in addition to indels. The mutational combinations revealed three haplotypes in the cultivars, which are the main haplotypes in the wild cherry populations. Chloroplast DNA diversity in wild cherry is higher (16 haplotypes) than in sweet cherry cultivars (three haplotypes). The probable wild origin of the sweet cherry cultivars in the maternal line, on the basis of haplotypic similarity, was discussed. |
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| Keywords: | Prunus avium sweet cherry cultivars wild cherry cpDNA diversity PCR-RFLP |
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