| 多重PCR结合DHPLC方法检测番茄中转基因成分 |
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| 引用本文： | 白月,才华,栾凤侠,关学佳.多重PCR结合DHPLC方法检测番茄中转基因成分[J].作物杂志,2011(2):28-31. |
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| 作者姓名： | 白月 才华 栾凤侠 关学佳 |
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| 作者单位： | 1. 黑龙江出入境检验检疫局,150001,黑龙江哈尔滨
2. 东北农业大学,150030,黑龙江哈尔滨 |
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| 基金项目： | 2011年国家质检总局项目，黑龙江省科技厅青年基金 |
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| 摘 要： | 应用多重PCR结合变性高效液相色谱(DHPLC)技术,检测转基因番茄华番1号中的转基因成分。对番茄中特异的PG内源基因、华番1号中含有的CAMV35S、NOS和NPTII外源基因进行多重PCR扩增,将扩增产物测序验证。利用DH-PLC分离多重PCR产物,建立了多重PCR-DHPLC技术检测转基因番茄的方法;将样品进行稀释,确定了该方法的检测灵敏度。试验结果表明,所建立的多重PCR-DHPLC方法操作简便,快速准确,能够同时检测转基因番茄4个内、外源基因,检测限达0.5ng/μL,可用于番茄中转基因成分的检测。
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| 关 键 词： | 转基因番茄 多重PCR 变性高效液相色谱 检测 |
Detection of Genetically Modified Components in Tomato by Multiplex PCR and DHPLC |
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| Bai Yue,Cai Hua,Luan Fengxia,Guan Xuejia.Detection of Genetically Modified Components in Tomato by Multiplex PCR and DHPLC[J].Crops,2011(2):28-31. |
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| Authors: | Bai Yue Cai Hua Luan Fengxia Guan Xuejia |
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| Institution: | Bai Yue1,Cai Hua2,Luan Fengxia1,Guan Xuejia1(1Heilongjiang Entry-Exit Inspection and Quarantine Bureau,Harbin 150001,Heilongjiang,2Northeast Agriculture University,Harbin 150030,China) |
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| Abstract: | Multiplex PCR-DHPLC method were used for detecting genetically modified components in Huafan NO.1 tomato.CAMV35S,NOS and NPTII genes were detected by multiplex PCR.The endogenesis PG gene was also be amplified.The multiplex PCR fragments were sequenced by company and separated by DHPLC.Various grads of samples were used for the sensitivity testing.The result showed that the multiplex PCR-DHPLC separation method was proposed for the simultaneous detection of 4 genes in Huafan NO.1 tomato.The limit of detecti... |
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| Keywords: | Transgenic tomato Multiplex PCR DHPLC Detection |
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