| 种植模式对苜蓿固氮和固氮产物转移的影响 |
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| 引用本文： | 姚允寅,陈明,张希忠.种植模式对苜蓿固氮和固氮产物转移的影响[J].核农学报,1993,7(3):157-162. |
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| 作者姓名： | 姚允寅 陈明 张希忠 |
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| 作者单位： | 中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所 北京 100094 ,北京 100094 ,北京 100094 |
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| 摘 要： | 田间小区试验研究了不同种植模式下苜蓿的共生固氮贡献,并利用~(15)N同位素示踪技术评估了苜蓿的％Ndfa和Ndfa,以及与之混作生长的牛尾草植株中来自苜蓿固氮产物的转移量。研究表明,豆科与禾本科牧草混作对发挥草地的优势有一定影响,混作条播在干草产量、全氮产量、％Ndfa和Ndfa等方面均优于间作与混作撒播模式,且高于单作苜蓿与牛尾草的平均值。用~(15)N同位素稀释法与~(15)N天然丰度法评估苜蓿的％Ndfa与Ndfa值时,无明显差异(P<0.05),前者还能准确测出混种牛尾革植株中的固氮产物转移量,后者则大大低估,甚至不能测出固氮产物转移。
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| 关 键 词： | ~(15)N同位素稀释法 ~(15)N天然丰度法 种植模式 固氮 转移 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
EFFECT OF PLANTING PATTERNS ON DINITROGEN FIXATION OF ALFALFA AND TRANSFER OF N FIXED |
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| Abstract: | Contribution of symbiotical nitrogen fixation of alfalfa grown with different planting patterns was studied in a field experiment.%Ndfa and Ndfa in alfalfa and N transferred from alfalfa in meadow fescue were examined by 2 kinds of 15N tracer techniques.The superiority of mixed culture of legumes with grasses to monoculture was influenced by planting patterns.Biomass in a mixed culture was related to proportion of alfalfa in it.The proportion of alfalfa was in close relationship not only with ratio of their seeds,but also with planting patterns.Row seeding in mixed seeds was better than broadcasting or intercropping in hay yield,total N yield and %Ndfa and Ndfa.It was also higher than the average of corresponding item of alfalfa and meadow fescue in monoculture each equal area.There was no significantly difference (P<0.05) between estimates of %Ndfa or Ndfa of alfalfa grown in field using two methods,15N isotope dilution method and natural 15N abundance method.N in meadow fescue transferred from alfalfa could be accurately determined by 15N isotope di.fution method,but 15N abundance method gave underestimates,even could not examined N in grasses transferred from associated legumes. |
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| Keywords: | 15N isotope dilution method natural 15N abundance method planting pattern dinitrogen fixation transfer |
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